Glucose oxidase, uricase, and urease were immobilized on the interior surface of activated polyamide tubing. The shelf-life of such enzyme bearing tubes was at least six months. The tubes were used for continuous-flow analysis of glucose, uric acid, and urea with conventional systems and with hybrid micro-scale systems in which modules of different manufacture were combined. The length of enzym...

In this research, we have demonstrated a one-step electrochemical deposition of dendritic gold nanostructures (DGNs) on graphite rod (GR) electrode without any template, seeds, surfactants, or stabilizers. Three methods, namely, constant potential amperometry (CPA), pulse amperometry, and differential voltammetry, were used for DGN synthesis GR further application in enzymatic glucose biosensor...

Fungal pyranose oxidase is a flavoenzyme whose preferred substrate among several monosaccharides is D-glucose. After a comprehensive analysis of conserved features in a structure-based multiple sequence alignment of homologous proteins, we could classify this enzyme into the GMC oxidoreductase family. The identified homology also suggests a three-dimensional protein structure similar to the fun...

A novel hybrid material that mimics NAD(P)H oxidase was designed and tested for the in situ regeneration of assorted enzyme cofactors, as reported by Ana Beloqui co-workers their Communication (e202206926). This fabricated controlled assembly glucose hemin catalysts supported a functional nanogel allocates (bio)catalysts different compartments. The synthesis procedure allows fabrication heterog...

An enzyme glucose microsensor using a glucose oxidase-immobilized porous carbon/Teflon composite microelectrode was developed. The microsensor was fabricated by etching a platinum microelectrode (platinum, radius of 25 and 50 micrometers) in hot aqua regia to create a cavity at the tip and then packing a porous carbon/Teflon composite, which was made from acetylene black and Teflon emulsion, in...

Glucose oxidase catalyzes the formation of metallic gold particles in immediate proximity of the protein from gold (III) chloride in the absence of any other catalytic or reductive substrates. The protein-mediated gold reduction reaction leads to size-controllable gold particle formation and concomitant association of the enzyme in an electrically conductive metallic template. Such an enzyme im...

Molecular nanoprobes with intrinsic enzyme-like activity represent a new wave of technology for rapid and sensitive detection molecular targets. This work reports synthesis characterization novel well-dispersed europium-doped ceria nanocrystals (EuCe NCs) self-integrated catalytic fluorescence sensing functions. The NCs have an average size ∼5 nm exhibit bright stable more than 6 months in aque...

Glucose oxidase (GO) activity is generally restricted to glucose and is susceptible to inactivation by H2O2. By comparison, the Y300A variant of gluco-oligosaccharide oxidase (GOOX) from Sarocladium strictum showed broader substrate range and higher H2O2 stability. Specifically, Y300A exhibited up to 40 times higher activity on all tested sugars except glucose, compared to GO. Moreover, fusion ...

Glucose oxidase is an important enzyme hydrolyzing for its hydrolyzing activity on glucos. It possesses and has a wide board of applications in different industries such as bakery, pharmaceutical, plant pathology and biosensors. In this study, yeast (Saccharomyces cerevisiae) was transformed successfully by the glucose oxidase gene (gox) obtained from Penicillium funicolusum. The secreted gluco...

Microelectrodes have been used for over 30 years of glucose biosensor history. Two different types of glucose biosensors were developed using 176μm and 25μm diameter Pt wire cylindrical microelectrodes. The 176 μm Pt wire type microelectrodes were modified with non-conductive polymers and glucose oxidase enzyme. The lifetime of biosensors was over 50 days and had negligible response to interfer...