In this work, twelve analogues of piperidine alkaloids (-)-cassine and (-)-spectaline were synthesized, as well the racemic forms these natural products. The compounds evaluated for their inhibition electric eel acetylcholinesterase (AChEee) human butyrylcholinesterase (BChEhu) by on-flow mass-spectrometry-based dual-enzyme assay, mechanisms most potent also determined. Our results showed a pre...

background: myeloperoxidase (mpo), which is abundantly expressed in neutrophils, catalyzes the formation of a number of reactive oxidant species. however, evidence has emerged that mpo-derived oxidants contribute to tissue damage and initiation and propagation of inflammatory diseases, particularly, cardiovascular diseases. therefore, studying the regulatory mechanisms of the enzyme activity is...

Natural products have a key role in drug discovery pharmacology and medicine. Prunus spinosa L. (blackthorn) grown Çanakkale province western Turkey, is known as medicinal plant, rich source of biologically active compounds such phenolics, flavonoids anthocyanidins. The flower fruit extracts the plant are subjects many studies, but they usually lack details its potential for bio-inhibition stud...

A radioinhibitor binding assay and an enzyme inhibition assay have been developed to measure plasma levels of CGP 38 560, a potent human renin inhibitor. The detection limit of the assays was between 0.5 and 1 pmol/ml. There was a good correlation (r = 0.989) between the two assays for the measurement of human plasma spiked with CGP 38 560 in concentrations from 1.9 nM to 12 microM. Intra-assay...

background while hepatitis e virus (hev) mostly causes self-limited disease in general population, it is more severe in pregnant women. objectives this study aimed to investigate the seroprevalence of anti-hev igg among a population of pregnant women in west azerbaijan of . patients and methods one hundred thirty six pregnant women referred to urban health centers of urmia for pursuing pregnanc...

materials and methods a total of 228 fecal samples were collected from residents of rural areas of hamadan, west of iran. each fecal sample was divided into two parts, one kept frozen at -20˚c for ag-capture elisa and the other in 10% formalin for the ap staining method. cryptosporidium ag-detection elisa procedure was performed according to the manual of the manufacturer. the preserved samples...