Enzyme kinetics studies normally focus on the initial rate of enzymatic reaction. However, the manual operation of steps of the conventional enzyme kinetics method has some drawbacks. Errors can result from the imprecise time control and time necessary for manual changing the reaction cuvettes into and out of the detector. By using the automatic flow-based analytical systems, enzyme kinetics st...

The statistics of steps and dwell times in reversible molecular motors differ from those of cycle completion in enzyme kinetics. The reason is that a step is only one of several transitions in the mechanochemical cycle. As a result, theoretical results for cycle completion in enzyme kinetics do not apply to stepping data. To allow correct parameter estimation, and to guide data analysis and exp...

A 'first-passage-time' analysis is applied to enzyme kinetics. It is shown that the residence times determined in this way are directly related to the steady-state parameters and are particularly useful in analysis of isotopic exchange. A simple linear means is used for the calculation of these residence times that makes this method easily applicable to the numerical evaluation of complex model...

A novel method of estimating enzyme kinetic parameters is presented using the Lambert omega function coupled with nonlinear regression. Explicit expressions for the substrate and product concentrations in the integrated Michaelis-Menten equation were obtained using the omega function which simplified kinetic parameter estimation as root-solving and numerical integration of the Michaelis-Menten ...