نتایج جستجو برای: escherichia coli

تعداد نتایج: 153418  

2013
Juhui Kim Eunyoung Ro Kisun Yoon

Growth kinetics for Escherichia coli O157:H7 in perilla leaves were compared to those of pathogenic E. coli strains, including enteropathogenic (EPEC), enterotoxigenic (ETEC), enteroinvasive (EIEC) and other enterohemorrhagic (EHEC) at 13, 17, 24, 30 and 36 °C. Models for lag time (LT), specific growth rate (SGR) and maximum population density (MPD) as a function of temperature were developed. ...

Journal: :Journal of clinical microbiology 2002
Jun Yatsuyanagi Shioko Saito Hiroyasu Sato Yoshimichi Miyajima Ken-Ichi Amano Katsuhiko Enomoto

Virulence characteristics of diarrheal outbreak-associated Escherichia coli O55:NM, O126:NM, and O111:NM were examined. The E. coli O55:NM strains were atypical enteropathogenic E. coli (EPEC), while the E. coli O126:NM and O111:NM strains should be classified as enteroaggregative E. coli (EAggEC). The contributions of EPEC and EAggEC to the human disease burden in Japan might be significantly ...

2016
Thomas Rice Noreen Quinn Roy D. Sleator Brigid Lucey

The recent paradigm shift in infectious disease diagnosis from culture-based to molecular-based approaches is exemplified in the findings of a national study assessing the detection of verotoxigenic Escherichia coli infections in Ireland. The methodologic changes have been accompanied by a dramatic increase in detections of non-O157 verotoxigenic E. coli serotypes.

2016
Adriana Cabal María García-Castillo Rafael Cantón Christian Gortázar Lucas Domínguez Julio Álvarez

Etiological diagnosis of diarrheal diseases may be complicated by their multi-factorial nature. In addition, Escherichia coli strains present in the gut can occasionally harbor virulence genes (VGs) without causing disease, which complicates the assessment of their clinical significance in particular. The aim of this study was to detect and quantify nine VGs (stx1, stx2, eae, aggR, ehxA, invA, ...

Journal: :Memorias do Instituto Oswaldo Cruz 2007
Silvia Y Bando Luiz R Trabulsi Carlos A Moreira-Filho

The genetic relationship among the Escherichia coli pathotypes was investigated. We used random amplified polymorphic DNA (RAPD) data for constructing a dendrogram of 73 strains of diarrheagenic E. coli. A phylogenetic tree encompassing 15 serotypes from different pathotypes was constructed using multilocus sequence typing data. Phylogram clusters were used for validating RAPD data on the clona...

2015
Susan R. Leonard David W. Lacher Keith A. Lampel

We report here the draft genome sequences of enteroinvasive Escherichia coli (EIEC) O124:H30 strain M4163 isolated from imported French cheese and EIEC O143:H26 strain 4608-58. The assembled data determined that both strains contain multiple copies of the ipaH gene, as well as the pINV A form of the invasion plasmid.

Journal: :Applied and environmental microbiology 2013
Jatinder P S Sidhu Warish Ahmed Leonie Hodgers Simon Toze

Escherichia coli isolates (n = 300) collected from six sites in subtropical Brisbane, Australia, prior to and after storm events were tested for the presence of 11 virulence genes (VGs) specific to diarrheagenic pathotypes. The presence of eaeA, stx(1), stx(2), and ehxA genes specific for the enterohemorrhagic E. coli (EHEC) pathotype was detected in 56%, 6%, 10%, and 13% of isolates, respectiv...

Journal: :journal of paramedical sciences 0
fatemeh moazen department of pharmaceutical biotechnology and isfahan pharmaceutical sciences research center, school of pharmacy and pharmaceutical sciences, isfahan university of medical sciences, isfahan, iran. fatemeh shafiee department of pharmaceutical biotechnology and isfahan pharmaceutical sciences research center, school of pharmacy and pharmaceutical sciences, isfahan university of medical sciences, isfahan, iran. marjan doostan department of pharmaceutical biotechnology and isfahan pharmaceutical sciences research center, school of pharmacy and pharmaceutical sciences, isfahan university of medical sciences, isfahan, iran. hamid mir mohammad sadeghi department of pharmaceutical biotechnology and isfahan pharmaceutical sciences research center, school of pharmacy and pharmaceutical sciences, isfahan university of medical sciences, isfahan, iran. mohammad rabbani department of pharmaceutical biotechnology and isfahan pharmaceutical sciences research center, school of pharmacy and pharmaceutical sciences, isfahan university of medical sciences, isfahan, iran.

the µ (mu) opioid receptors, which mediate the effects of morphine, are widely distributed in brain. the purpose of this study was to design a simple expression system for rat µ-receptor in escherichia coli (bl21). in this laboratory study, rat µ-receptor cdna was isolated from pcdna3 vector using xba1 and hind3 restriction enzymes. pet-15b was digested by nco1 restriction enzyme. µ-receptor cd...

Journal: :jundishapur journal of microbiology 0
muhammad sohail department of microbiology and molecular genetics, quaid-e-azam campus, university of the punjab, lahore-54590, pakistan mohsin khurshid college of allied health professionals, directorate of medical sciences, government college university, faisalabad, pakistan; college of allied health professionals, directorate of medical sciences, government college university, allama iqbal road, faisalabad-38000, pakistan. tel: +92-3334301513, fax: +92-419201227 hafiz ghulam murtaza saleem department of microbiology and molecular genetics, quaid-e-azam campus, university of the punjab, lahore-54590, pakistan hasnain javed department of microbiology and molecular genetics, quaid-e-azam campus, university of the punjab, lahore-54590, pakistan abdul arif khan department of pharmaceutics, college of pharmacy, king saud university, riyadh, saudi arabia

conclusions our results showed that broad-spectrum antibiotics such as imipenem, meropenem, fosfomycin, cefoperazone/sulbactam, and vancomycin would be the first line and the most effective drugs for the empirical treatment of urinary tract pathogens due to their higher resistance rates against other drugs like cephalexin, cephradine, ciprofloxacin, levofloxacin, and norfloxacin. patients and m...

Journal: :jundishapur journal of microbiology 0
zohreh hojati department of biology, faculty of sciences, university of isfahan, isfahan, ir iran behnam zamanzad department of microbiology and immunology, cellular and molecular research center, faculty of medicine, shahrekord university of medical sciences, shahrekord, ir iran morteza hashemzadeh cellular and molecular research center, faculty of medicine, shahrekord university of medical sciences, shahrekord, ir iran razieh molaie department of microbiology and immunology, cellular and molecular research center, faculty of medicine, shahrekord university of medical sciences, shahrekord, ir iran abolfazl gholipour department of microbiology and immunology, cellular and molecular research center, faculty of medicine, shahrekord university of medical sciences, shahrekord, ir iran; department of microbiology and immunology, cellular and molecular research center, faculty of medicine, shahrekord university of medical sciences, shahrekord, ir iran. tel: +98-9137046656, fax: +98-3813334911

conclusions the results of this study indicated that more than 90% of e. coli isolates harbored the fimh gene. the high binding ability of fimh could result in the increased pathogenicity of e. coli; thus, fimh could be used as a possible diagnostic marker and/or vaccine candidate. materials and methods a total of 140 isolated e. coli strains from patients with uti were identified using biochem...

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