enterohemorrhagic escherichia coli (ehec) of the o157:h7 serotype is a worldwide zoonotic pathogen responsible for the majority of severe cases of human ehec disease. the aim of the present study was to investigate the prevalence of e. coli o157: h7/nm in raw meat samples from two provinces of iran. during a period from march 2010 to march 2011. two hundred and ninety five raw meat samples were...

background: extended spectrum beta lactamases (esbls) are an important cause of transferable multidrug resistance (mdr) in gram-negative bacteria. the most described esbl genes are generally found within integron-like structures as mobile genetic elements. the aim of this study was to identify the accompanying of class 1 integrons and esbls in the mdr e. coli isolates. methods: susceptibility t...

BACKGROUND/AIM Helicobacter pylori is a major health problem. One of the therapeutic approaches is administration of antibody against H. pylori. The methylotrophic Pichia pastoris is a suitable host for expression of recombinant antibody fragments. The aims of this study were the expression and the evaluation of camelid nanobody in the yeast Pichia pastoris. MATERIALS AND METHODS The camelid-...

Canine parvovirus (CPV) appears to be endemic in almost all populations of wild and domesticated dogs. It causes serious contagious enteric disease. The VP2 protein of CPV is a major capsid protein and plays an important role in the host immune response. In the present study, the recombinant VP2 was expressed in Escherichia coli (E. coli) using the pBAD expression system. Virus DNA from the inf...

background and objectives: diarrheagenic escherichia coli (dec) is an emerging agent among pathogens that causes diarrhea. studies showed that diarrheagenic e. coli such as enterohaemorrhagic e. coli (ehec), enteroaggregative e. coli (eaec), enteropathogenic e. coli (epec), enteroinvasive e. coli (eiec), enterotoxigenic e. coli (etec), diffusely adhering e. coli (daec) and shiga toxin producing...

Antimicrobial peptides (AMPs) can cause lysis of target bacteria by directly inserting themselves into the lipid bilayer. This killing mechanism confounds the identification of the intracellular targets of AMPs. To circumvent this, we used a shuttle vector containing the inducible expression of a human cathelicidin-related AMP, LL-37, to examine its effect on Escherichia coli TOP10 under aerobi...

NF-kappaB is a crucial mediator of macrophage inflammatory responses, but its role in the context of pathogen-induced adaptive immune responses has yet to be elucidated. Here, we demonstrate that classical NF-kappaB activation delays phagocytosis-induced cell death (PICD) in Raw 264.7 and bone marrow-derived macrophages (BMDMs) upon ingestion of bacteria from the Escherichia coli laboratory str...

NFB is a crucial mediator of macrophage inflammatory responses, but its role in the context of pathogeninduced adaptive immune responses has yet to be elucidated. Here, we demonstrate that classical NFB activation delays phagocytosis-induced cell death (PICD) in Raw 264.7 and bone marrow-derived macrophages (BMDMs) upon ingestion of bacteria from the Escherichia coli laboratory strain Top10. By...

background: leukemia inhibitor factor (lif) is a very important pleiotropic cytokine which belongs to interleukin-6 (il-6) family. lif exerts multiple effects on different types of cells and tissues with numerous regulatory effects in vivo and in vitro. it is a lymphoid factor, which performs a number of activities including cholinergic neuron differentia­tion, control of stem cell pluripotency...