We have used an immunoperoxidase technique utilizing a monoclonal antibody to the estradiol receptor to identify immunoreactive estradiol receptor in breast carcinomas and have examined the relationship between the immunoreactive estradiol receptor and response to therapy in patients with advanced breast cancer. Fifty-six patients were found to be assessable for response to endocrine therapy. T...

the role of antioxidants in prevention and treatment of cancers have been reported by several studies. in our investigation we studied the effects of ascorbic acid, ?-tocopherol, and sodium selenite on proliferation of two breast cancer cell lines: t47d (estrogen-receptor positive) and mda-mb-231 (estrogen-receptor negative). we also used 17-?-estradiol as positive control for proliferation of ...

BACKGROUND The endocrinology of skeletal muscle is highly complex and many issues about hormone action in skeletal muscle are still unresolved. Aim of the work is to improve our knowledge on the relationship between skeletal muscle and 17β-estradiol. METHODS The skeletal muscle cell line C2C12 was treated with 17β-estradiol, the oxytocin peptide and a combination of the two hormones. The mRNA...

The binding of estradiol--receptor complexes of mouse uterine cytosol to oligodeoxynucleotide celluloses is inhibited by the sulfonated polyaromatic dye Cibacron blue F3GA. The dye does not have any effect on the estradiol binding site. Additon of the dye to preformed estradiol--receptor--oligo(dT)-cellulose complex results in the release of estradiol--receptor. The inhibition of binding is com...

Introduction: The 17β-estradiol acts as a neurosteroid in the brain and modulates nociception by binding to the estrogen receptors and also by allosteric interaction with other membrane-bound receptors like glutamate receptors. Paragigantocellularis lateralis nucleus (LPGi) is one of the important brain regions implicated in the pain modulation. So, this study was designed to evaluate the ...

Nuclear estrogen binding was characterized in IIM-1, a malignant hamster melanoma cell line transplanted into male and female athymic mice following acute, subchronic, and chronic injection of estradiol. Nuclear binding was saturable, of high affinity ( IO'" M ') and readily soluble in low salt buffer. Saturation analyses revealed that (3H]estradiol in excess of 5.0 IIMapparently bound to a sec...

Estradici and progesterone receptors were assayed in tumors from 79 patients with primary colorectal and 56 patients with stomach adenocarcinomas. Eighteen of 79 colorectal cancers contained estradiol receptor, while 34 specimens were positive for progesterone receptor. In stomach cancer, the positive sam ples were 8 for estradiol and 14 for progesterone receptors. In both types of tumors, the ...

Nuclear estrogen binding was characterized in IIM-1, a malignant hamster melanoma cell line transplanted into male and female athymic mice following acute, subchronic, and chronic injection of estradiol. Nuclear binding was saturable, of high affinity ( IO'" M ') and readily soluble in low salt buffer. Saturation analyses revealed that (3H]estradiol in excess of 5.0 IIMapparently bound to a sec...

Introduction: 17β-estradiol modulates nociception by binding to estrogenic receptors and also by allosteric interaction with other membrane-bound receptors like glutamate and GABAA receptors. Beside its autonomic functions, paragigantocellularis lateralis (LPGi) nucleus is also involved in pain modulation. The aim of the current study was to investigate the role of the intracellular estrogen...