Stevia rebaudiana is a herbaceous perennial plant with great global demand due to its beneficial steviol glycosides(SGs) content. Current conventional breeding technique unable cater the need for more S. planting materials. Therefore, an improved in vitro shoot regeneration protocol was developed by using chitosan. The highest fresh weight of (0.586 ± 0.176 g/explant), dry (0.056 0.02 g/explant...

Objective: To compare the characteristics of human gingival fibroblasts (hGFs) obtained by tissue-explant and enzyme-digestion methods. Methods: The hGFs were isolated using tissue explants and enzyme digestion methods and identified by immunocytochemical analysis. Cell-surface molecules were detected by flow cytometry analysis. The colony-forming activity was examined by CFU-F assay. Oil red O...

BACKGROUND Insulin-like growth factor 1 (IGF-1) activates prosurvival pathways and improves postischemic cardiac function, but this key cytokine is not robustly expressed by cultured human cardiac stem cells. We explored the influence of an enhanced IGF-1 paracrine signature on explant-derived cardiac stem cell-mediated cardiac repair. METHODS AND RESULTS Receptor profiling demonstrated that ...

OBJECTIVE The aim of this study is the histological and immunohistochemical evaluation of ex vivo produced oral mucosal equivalents using keratinocytes cultured by direct explant technique. MATERIAL AND METHODS Oral mucosa tissue samples were obtained from the keratinized gingival tissues of 14 healthy human subjects. Human oral mucosa keratinocytes from an oral mucosa biopsy specimen were di...

Objective The purpose of this study was to investigate the effects of estradiol on the expression of hypoxia-inducible factor (HIF)-1α and the differentiation of trophoblasts in human first trimester villous explant cultures. Methods Villous explant cultures were established from first trimester human placentas (6-8 weeks of gestation, n=3). Normal villous tissues were explanted on Matrigel a...

PURPOSE A previous report has described an ocular surface reconstruction method involving the use of cultivated corneal epithelium derived from limbal explants. In the current study, a new culture system was developed involving the in vitro propagation on amniotic membrane (AM) of epithelial cells from enzymatically dissociated limbal epithelium. The purpose of this new method is to produce a c...

PURPOSE The clinical success of treating corneas with total limbal stem cell deficiency using limbal biopsy explants cultured on intact amniotic membrane (iAM) relies on ex vivo expansion of limbal epithelial progenitor cells. However, the ultimate fate of limbal epithelial progenitor cells in the explant remains unclear. METHODS Human limbal explants were cultured on iAM for 2 weeks and then...