Figure 3: Fitting of representative liver voxels from the two techniques in Patient 2 of Fig. 1. T2*-IDEAL, based on complex data and weighted least squares, accurately captures the T2* decay, whereas the multi-echo exponential fitting demonstrates over-estimation of the T2*. Figure 1: representative results from two patients. Water and fat images from the T2*-IDEAL technique are shown in addit...

We present MEMLET (MATLAB-enabled maximum-likelihood estimation tool), a simple-to-use and powerful program for utilizing maximum-likelihood estimation (MLE) for parameter estimation from data produced by single-molecule and other biophysical experiments. The program is written in MATLAB and includes a graphical user interface, making it simple to integrate into the existing workflows of many u...

Several important aspects of fluorescence decay analysis are addressed and tested against new experimental measurements. A simulated-annealing method is described for deconvoluting the instrument response function from a measured fluorescence decay to yield the true decay, which is more convenient for subsequent fitting. The method is shown to perform well against the conventional approach, whi...