Background and Methods: Paratuberculosis is a chronic disease of ruminant, caused by Mycobacterium avium subsp. paratuberculosis (Map), clinically infected animals produce high level of antibodies in blood and shed detectable amount of Map organisms in feces. Several serological and molecular tests are utilized for detection of antibodies or DNA of the organism in clinical samples. Present stud...

abstract         background and objective: paratuberculosis has been repeatedly reported from iranian ruminant herds. the extrem fastidious nature of mycobacterium avium subspecies paratuberculsos hinders genomic diversity studies of the pathogen. short sequence repeat analysis is one of the genome-based approches recently developed to overcome this difficulty. in this study we describe the app...

Giannico G, Cortes P, Baccora MH, Hassett C, Taube DW, Yee J. Glibenclamide prevents increased extracellular matrix formation induced by high glucose concentration in mesangial cells. Am J Physiol Renal Physiol 292: F57–F65, 2007. First published August 8, 2006; doi:10.1152/ajprenal.00210.2006.—Other than stimulation of cell contractility, little is known about the potential metabolic effects i...

The objective of this study was to compare in situ and in vitro methods for determining rate and extent of forage digestion in the rumen of beef cattle. Duplicate polyester (10 × 20 mm) or ANKOM F57 filter bags containing 5.0 or 0.5 g of ground (2mm) bromegrass hay, respectively, were incubated in situ or in vitro (ANKOM Daisy Incubator) for 3, 6, 9, 12, 15, 18, 24, 36, and 96 hours. Bags were ...

abstract        background and objective: paratuberculosis is a chronic granulomatous enteritis of ruminants caused by mycobacterium avium subspecies paratuberculosis (map). this study aimed to characterize the genome of the map 316f strain.       methods: the map 316f strain was subjected to the pcr-f57 and pcr-is900 experiments in order to ensure its identity as map. this was followed by appl...