the latest micromanipulative technique, intracytoplasmic sperm injection (icsi), has been very successful in cases of severe male factor infertility. the main objective of this study is to report the results of our first experience with icsi in cases of male factor infertility, as well as patients who had failed their previous cycles with conventional in vitro fertilization (ivf). normal fertil...

Artificial oocyte activation to overcome failed fertilization after intracytoplasmic sperm injection (ICSI) in human oocytes typically employs Ca2+ ionophores to produce a single cytosolic Ca2+ increase. In contrast, recombinant phospholipase Czeta (PLCz) causes Ca2+ oscillations indistinguishable from thoseoccurring during fertilization, but remains untested for its efficacy in a scenario of I...

Artificial oocyte activation to overcome failed fertilization after intracytoplasmic sperm injection (ICSI) in human oocytes typically employs Ca(2+) ionophores to produce a single cytosolic Ca(2+) increase. In contrast, recombinant phospholipase Czeta (PLCζ) causes Ca(2+) oscillations indistinguishable from those occurring during fertilization, but remains untested for its efficacy in a scenar...

The mammalian zona pellucida contains several glycoproteins whose oligosaccharide moieties are known to play a key role in the interaction with spermatozoa. Since zona pellucida defects may represent one of the most likely causes of failed fertilization in human in-vitro reproduction, we have studied the carbohydrate composition and distribution over the human zona pellucida by means of lectins...

background: irradiation is one of the major causes of induced sperm dna damage. various studies suggested a relation between sperm dna damage and fertilization rate after intra-cytoplasmic sperm injection (icsi). objective: in this study, fertilization rate and premature chromosome condensation (pcc) formation after icsi of hamster oocytes with irradiated sperms from normal and oligosperm indiv...