BACKGROUND Heavy metals tend to bioaccumulate in living organisms, and their accumulation has been a major concern. As mammals are known to excrete heavy metals via their bile, it seems to be very promising to analyse metal burdens of parasites that infect the biliary tree such as liver flukes of the genus Fasciola. The present study was carried out to evaluate F. hepatica and F. gigantica as b...

A single-step multiplex PCR (here referred to as a duplex PCR) has been developed for simultaneous detection and diagnosis of Fasciola hepatica and F. gigantica. These species overlap in distribution in many countries of North and East Africa and Central and Southeast Asia and are similar in egg morphology, making identification from fecal samples difficult. Based on a comparative alignment of ...

Fascioliasis, or trematode infestation, is an important disease caused by Fasciola hepatica and Fasciola gigantica. Both species are hepatic parasites that affect humans. We have examined the zoonotic aspects of fascioliasis. A total of 80 fresh liver samples were collected from 67 Kedah-Kelantan crossbred cattle and 13 Murrah buffalo at 4 local abattoirs in Perak, Malaysia. The samples were ex...

Nucleotide sequences of ribosomal internal transcribed spacer (ITS1) and mitochondrial NADH dehydrogenase I (NDI) gene were analyzed to genetically characterize aspermic Fasciola forms in Korea. From the difference in ITS1 sequences, Korean flukes were divided into 3 haplotypes represented by Kor1, Kor2 and Kor1/2, which had nucleotides identical to F. hepatica, F. gigantica and those overlappe...

BACKGROUND In this study, we used both ITS1 and ITS2 for molecular identification of Fasciola species. METHODS The region between 18S and 28S of ribosomal DNA was used in PCR-RFLP method for molecular identification of Fasciola species. Ninety trematodes of Fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from Khorasan, East Azerbaijan, and...

objective(s): fascioliasis is a zoonotic parasitic disease caused by liver fluke species of fasciola hepatica and fasciola gigantica. differentiation of these two species, based on their morphological characteristics, is difficult. the current study aimed to use pcr-rflp assay to distinguish between f. hepatica and f. gigantica , based on profiles of rflp, produced by effect of endonucleases on...

The prevalence of helminthiasis was found to be 15.2 % in buffaloes. Out of 500 samples examined, 9.2 % and 5.4 % were infected with nematodes and trematodes respectively. Mixed infection was observed in 0.6 %. The chief helminths identified were: Toxocara vitulorum (3.6 %), Fasciola gigantica (3.2 %) Oesophagostomum radiatum (3.0 %) Strongyloides papillosus (2.4 %), Fasciola hepatica (2.2 %), ...

BACKGROUND Tropical fasciolosis caused by Fasciola gigantica infection is one of the major diseases infecting ruminants in the tropical regions of Africa and Asia including Thailand. Parasitological diagnosis of fasciolosis is often unreliable and possesses low sensitivity. Therefore, the detection of circulating parasite antigens is thought to be a better alternative for diagnosis of fasciolos...

fascioliasis is a worldwide parasitic disease in human and domestic animals. the causative agents of fascioliasis are fasciola hepatica and fasciola gigantica. in the recent years, fasciola resistance to drugs has been reported in the many of publications. fasciola spp has detoxification system including gst enzyme which may be responsible for its resistance. therefore , the aim of the study wa...

This study reports the early biochemical changes in plasma, comparative host-immune responses and parasite recovery data in Merino sheep during the first 10 weeks of infection with Fasciola gigantica and Fasciola hepatica. One group of sheep were uninfected, four groups of sheep received incremental challenge doses of F. gigantica metacercariae (50, 125, 225 and 400, respectively) and the sixth...