The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-h...

the ability of neurospora crassa fgsc 4335 in the biotransformation of hydrocortisone was investigated. the microorganism produced two major metabolites after incubation with the substrate for seven days. each microbial product was purified chromatographically and identified on the basis of spectral data. the products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (ii) and 11?-h...

eth-1: a thermosensitive allele of the Neurospora C T U S S ~ Sadenosylmethionine (AdoMet) synthetase gene that confers ethionine resistance, has been cloned and sequenced. Replacement of an aspartic amino acid residue (D48 + N4*), perfectly conserved in prokaryotic, fungal and higher eukaryotic AdoMet synthetases, was found responsible for both thermosensitivity and ethionine resistance confer...

The possibility that women produce new oocytes post-natally as part of the normal physiological function of the ovary is currently under investigation. Post-natal production of oocyte-like cells has been detected under experimental conditions in the mouse. Although these cells have many characteristics of oocytes, their potential to mature to fertilization-competence was unproven. Zou et al. (P...

Mating type tests in Neurospora crassa are an important way to characterize strains. Since most of the knock-out mutants developed as part of the functional genomics program (Colot et al., 2006) have little obvious phenotype we have undertaken to test the mating type of all of the knock-strains that are sent to the FGSC. Our original mating type test protocol is similar to that described by Smi...

Human female germline stem cells (FGSCs) have opened new opportunities for understanding human oogenesis, delaying menopause, treating infertility, and providing a new strategy for preserving fertility. However, the shortage of adult human ovaries tissues available impedes their future investigations and clinical applications. Here, we have established FGSC lines from scarce ovarian cortical ti...

The mainstream algorithms used for ship classification and detection can be improved based on convolutional neural networks (CNNs). By analyzing the characteristics of images, we found that difficulty in image lies distinguishing ships with similar hull structures but different equipment superstructures. To extract features such as superstructures, this paper introduces transformer architecture...

Genome defense likely evolved to curtail the spread of transposable elements and invading viruses. A combination of effective defense mechanisms has been shown to limit colonization of the Neurospora crassa genome by transposable elements. A novel DNA transposon named Sly1-1 was discovered in the genome of the most widely used laboratory "wild-type" strain FGSC 2489 (OR74A). Meiotic silencing b...

We report the discovery and validation of a set of single nucleotide polymorphisms (SNPs) between the reference Neurospora crassa strain Oak Ridge and the Mauriceville strain (FGSC 2555), of sufficient density to allow fine mapping of most loci. Sequencing of Mauriceville cDNAs and alignment to the completed genomic sequence of the Oak Ridge strain identified 19,087 putative SNPs. Of these, a s...

An enzyme preparation catalyzing the 2’-hydroxylation of thymidine was prepared from mycelial extracts of Neurospora crassa strain STA 4 (FGSC 2628) by chromatography on hydroxylapatite and DEAE-Sephadex A-50. The enzyme had an isoelectric point of 4.6 and a molecular weight of 47,000 (determined by gel filtration). The reaction product was identical with thymine riboside on ion exchange and th...