نتایج جستجو برای: fixed paraffin
تعداد نتایج: 207189 فیلتر نتایج به سال:
The molecular analysis of formalin-fixed and paraffin-embedded tissues has application in both investigative studies and clinical practice (1). It is now possible to analyze gene expression in formalin-fixed and paraffin-embedded tissues by in situ reverse transcriptase-polymerase chain reaction (in situ RT-PCR) (2). Degradation of RNA, however, can occur at various stages of tissue preparation...
PURPOSE Mutations in the epidermal growth factor receptor (EGFR) are associated with clinical and radiographic responses to EGFR tyrosine kinase inhibitors gefitinib and erlotinib. Currently available methods of EGFR mutation detection rely on direct DNA sequencing, which requires isolation of DNA from a relatively pure population of tumor cells, cannot be done on small diagnostic specimens, an...
AIMS To isolate RNA and DNA simultaneously from formalin fixed paraffin wax embedded tissue to assess the clonality of enteropathy associated T cell lymphomas and to analyse it in detail by a non-radioactive method of T cell receptor complementarity determining region 3 (CDR3) spectratyping. METHODS DNA and RNA were isolated simultaneously from formalin fixed paraffin wax embedded tissue bloc...
High-resolution melting (HRM) analysis is a novel tool for analysis of promoter methylation. The aim of the present study was to establish and validate HRM analysis for detection of promoter methylation on archival formalin-fixed paraffin-embedded tissues from colorectal cancer patients. We first evaluated HRM assays for O(6)-methylguanine-DNA methyltransferase (MGMT) and adenomatous polyposis ...
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