Recent developments in optical techniques for plasma diagnostics are critically reviewed. The primary emphasis is on plasma-induced emission and laser-induced fluorescence probes of radical and ionic concentrations and temperatures in low pressure discharges like those used in microelectronic fabrication. Other techniques such as optogalvanic, infrared, spontaneous and stimulated Raman, and mul...

A new and perspective addition to traditional fluorescent probes is the Au clusters (8−25 atoms) which can label proteins, rendering them extremely bright and photostable. In this paper, we show that albumins can quickly and effectively be labeled using microwave acceleration, which shortens the time of Au labeling from several hours to <30 s. Chromatography of Au proteins and FLIM (fluorescenc...

Sádecká J., Tóthová J. (2007): Fluorescence spectroscopy and chemometrics in the food classification − a review. Czech J. Food Sci., 25: 159–173. This review deals with the last few years’ articles on various fluorescence techniques (conventional, excitation-emission matrix, and synchronous fluorescence spectroscopy) as a tool for the classification of food samples. Chemometric methods as princ...

−− The interactions between Bovine Serum Albumin (BSA) and three flavonols, galangin, kaempferol and quercetin were studied by means of fluorescence spectroscopy. The fluorescence intensity of BSA exhibits remarkable decrease along with appreciable blue-shift of its maximum emission wavelength upon addition of the three compounds, respectively. The respective binding constant Kα and number of b...

We perform time-resolved fluorescence spectroscopy on clusters of plasmonic nanoantennas covered with a dye-polymer mixture. Dimer antenna structures were fabricated consisting of two interacting gold nanorods with varying lengths and interparticle separation. By combining four individual antennas into a cluster within a diffraction limited spot size, we can couple out half of the dye molecule ...

A tetraphenylethene (TPE)-decorated tripodal tris(urea) ligand was synthesized, which shows large emission enhancement when binding to an orthophosphate anion (PO4(3-)), but exhibits only weak or no fluorescence with other anions. The anion-binding and fluorescence properties were studied by X-ray crystal structure, NMR and fluorescence spectroscopy, and by DFT computations and the results demo...

Fluorescence spectroscopy is applied to study the influence of plasmon excitations in spherical Au nanoparticles on the optical properties of chlorophyll-containing light-harvesting complexes. The separation between the two nanostructures is controlled via silica layer with varied thickness. We observe strong increase of the emission intensity for a 12nm-thick spacer and the increase is accompa...

The fluorescence spectroscopy of 7-azaindole (In) incorporated in DNA oligonucleotides is investigated. Incorporation of In into DNA oligonucleotides is accomplished through standard solid-phase phosphoramidite chemistry. Fluorescence emission of the In chromophore shifts slightly to the red (from 386 nm to 388 nm) upon glycosylation at the N−1 position, but its relative fluorescence quantum yi...

background glucose is an essential element in the supply of body’s energy. in diseases such as diabetes, glucose increase is associated with disturbance in metabolism. cortisol is an important hormone in the regulation of glucose metabolism, and human serum albumin (hsa) is one of the most important glucose and cortisol transmitters in blood. interaction between albumin and these ligands could ...