Advances in RNA fluorescence in situ hybridization (RNA FISH) have allowed practitioners to detect individual RNA molecules in single cells via fluorescence microscopy, enabling highly accurate and sensitive quantification of gene expression. However, current methods typically employ hybridization times on the order of 2-16 hours, limiting its potential in applications like rapid diagnostics. W...

breast cancer is preservative of the genetic heterogeneous alterations. in the present investigation, fluorescence in situ hybridization (fish) was performed on the fresh primary tumours of 6 patients with infilterating ductal carcinoma. dna-probe for centromere of chromosome 3 (3cen) was applied and in the total analysis, the majority of interphases presented 1 signal (33.6%) which followed by...

The date palm (Phoenix dactylifera L.), 2n=36, is a dioecious long-lived monocotyledonous plant, which belongs to the family Arecaceae. It is one of the excellent candidate crops in arid and semiarid regions of the world. Recently, fluorescence in situ hybridization (FISH) has become a powerful and useful tool for the direct detection of specific DNA fragments in the genome. In this technique, ...

Patients with human epidermal growth factor receptor-2 (HER2)+ breast cancer are eligible for trastuzumab treatment; therefore, accurate assessment of HER2 status is essential. Until recently, only 2 methods were validated for determining the HER2 status of breast tumors in the routine diagnostic setting: immunohistochemical analysis and fluorescence in situ hybridization (FISH). Recently, brig...

Background A decrease in aneuploidy rate following a prolonged co-culture of human blastocysts has been reported. As co-culture is not routinely used in assisted reproductive technology, the present study aimed to evaluate the effect of the prolonged single culture on the rate of diploid cells in human embryos with aneuploidies. MaterialsAndMethods In this cohort study, we used fluorescence in ...

Our purposes were to perform a robust assessment of a new HER2 chromogenic in situ hybridization test and report on concordance of silver in situ hybridization (SISH) data with fluorescence in situ hybridization (FISH) data and on intraobserver and interlaboratory scoring consistency. HER2 results were scored from 45 breast cancers in 7 laboratories using the Ventana (Tucson, AZ) INFORM HER-2 S...

The gene encoding alpha fetoprotein (locus symbol Afp) was assigned to rat chromosome 14 at band p21-p22 using fluorescence in situ hybridization method. The present result suggests that there is a conserved syntenic group between human 4q11-q13, mouse 5F-G, and rat 14p21-p22.