Follicular dendritic cells (FDC) show homo- and heterocellular metabolic coupling through connexin 43 (Cx43) gap junctions and support B cell selection and maturation in germinal centers. In follicular lymphomas B cells escape apoptosis while FDC develop abnormally. Here we tested Cx43 channels in reactive FDC development and follicular lymphomas. In culture, the treatment of FDC-B cell cluster...

Ovaries, which provide a place for follicular development and oocyte maturation, are important organs in female mammals. Follicular development is complicated physiological progress mediated by various regulatory factors including microRNAs (miRNAs). To demonstrate the role of miRNAs in follicular development, this study analyzed the expression patterns of miRNAs in granulosa cells through inve...

BACKGROUND Estradiol (E2) receptors mediate E2 effects on cell proliferation and apoptosis under normal and pathological conditions. However, the mechanisms involved in E2 signaling are not completely understood. The objectives in this study were to evaluate the expression of estrogen receptors (ESRs) during follicular selection in cattle, and the effect of intrafollicular injection of fulvestr...

objective: sera from 186 sheep (93 rams and 93ewes) from tabriz, north-west of iran were evaluated for antibodies to toxoplasma gondii using a commercial elisa kit. methods: the animals were divided into two age groups (< 2 years, and > 2 years). results: antibodies to t. gondii were found in 13 of 186 sheep (6.98%). infection rate in ewes was higher than rams but this difference was not signif...

A review is given about follicular populations and aspects of follicular development in cattle. Ovaries of cattle contain two different pools of follicles, the non-growing pool and the growing pool. Entry of primordial follicles into the growth phase occurs throughout the reproductive life. Once follicles are recruited to grow, they are destined to undergo atresia or ovulation. Growth of obliga...

High-affinity antibodies are critical for host protection and underlie successful vaccines. The generation of such antibodies requires T cell–dependent help, which mediates germinal center reactions in which mutation and selection of B cells occurs. Using an interleukin 4–reporter system, we show here that CD4+ follicular helper T cells constituted essentially all of the cytokine-secreting T ce...

Nonsteroidal biochemical markers of oocyte maturity could presumably aid in oocyte selection and in the timing of insemination for in vitro fertilization. We assessed the usefulness of six potential markers found in follicular fluid. We used kinetic immunonephelometry to measure concentrations of alpha 1-antitrypsin, alpha 2-macroglobulin, antithrombin III, ceruloplasmin, fibrinogen, and plasmi...

ABSTRACT The objective of this study was to quantify the superovulatory response and embryo production Brazilian Bergamasca sheep evaluate link follicular condition before treatment, as a reference for selection donors with potential superovulation. Follicular population twenty-three evaluated by ultrasound during metestrus phase estrous cycle divided into groups low, medium high population. Su...

The size of ovarian follicular population evaluated by direct antral follicular count or endocrine markers can help determine the success of reproductive biotechnologies in cattle. However, although highly repeatable within animal, the antral follicular population (AFP) appears to be greatly variable across individuals. Therefore, laboratory methods that reliably predict AFP could have a signif...

background: the differential efficacy between long gnrh agonist with antagonist can partly be due to the preexisting differences in the early antral follicles before ovarian stimulation. objective: to compare the effect of pretreatment by estradiol with gnrh antagonist on antral follicular size coordination and basal hormone levels in gnrh antagonist protocol. materials and methods: on cycle da...