It was found that S. meliloti strain SmA818, which is cured of pSymA, could not grow on defined medium containing only formate and bicarbonate as carbon sources. Growth experiments showed that Rm1021 was capable of formate/bicarbonate-dependent growth, suggesting that it was capable of autotrophic-type growth. The annotated genome of S. meliloti Rm1021 contains three formate dehydrogenase genes...

Formate can differentiate between hyperhomocysteinemia due to impaired remethylation and impaired transsulfuration. Am J Physiol Endocrinol Metab 301: E000-E000, 2011. First published September 20, 2011; 10.1152/ajpendo.00345.2011.-We carried out a (1)H-NMR metabolomic analysis of sera from vitamin B(12)-deficient rats. In addition to the expected increases in methylmalonate and homocysteine (H...

Thiobacillus novellus was able to grow with oxalate, formate, formamide, and methanol as sole sources of carbon and energy. Extensive growth on methanol required yeast extract or vitamins. Glyoxylate carboligase was detected in extracts of oxalate-grown cells. Ribulose bisphosphate carboxylase was found in extracts of cells grown on formate, formamide, and thiosulfate. These data indicate that ...

PURPOSE The objective of this study was to perform a preliminary evaluation of the ocular and systemic safety of calcium formate, a dietary calcium supplement for prevention and management of osteoporosis. Although formate is an endogenous product of metabolism, high concentrations are associated with toxicity during methanol overdose. METHODS In this prospective clinical trial, 12 healthy wo...

Formate stimulates sodium chloride and fluid reabsorption in kidney proximal tubule; however, the exact cellular mechanism of this effect remains unknown. We hypothesized that the primary target of formate is the apical Na(+)/H(+) exchanger. Here, we demonstrate that formate directly enhances the apical Na(+)/H(+) exchanger (NHE3) activity in mouse kidney proximal tubule. In the absence of CO(2...

Formate stimulates sodium chloride and fluid reabsorption in kidney proximal tubule, however, the exact cellular mechanism of this effect remains unknown. We hypothesized that the primary target of formate is the apical Na/H exchanger. Here we demonstrate that formate directly enhances the apical Na/H exchanger (NHE3) activity in mouse kidney proximal tubule. In the absence of CO2/HCO3, additio...

A facile, convenient, green and environmentally benign one-pot three-component synthesis of poly-functionalized 4H-pyrans and 4H-chromenes is described. A basic ionic liquid 2-(2-hydroxyethoxy)ethaneammonium formate was found to be an efficient catalyst or catalytically active solvent for the synthesis of those important classes of heterocyclic compounds.

Pyruvate formate-lyase activating enzyme generates a stable and catalytically essential glycyl radical on G(734) of pyruvate formate-lyase via the direct, stereospecific abstraction of a hydrogen atom from pyruvate formate-lyase. The activase performs this remarkable feat by using an iron-sulfur cluster and S-adenosylmethionine (AdoMet), thus placing it among the AdoMet radical superfamily of e...

Two different possible mechanisms of water gas shift reaction including formate and redox mechanisms on the Ag5 cluster were investigated using DFT computations. All the elementary steps involved in both mechanisms were considered. It was observed that dissociation of H2Oads and OHads, as well as formation of CO2(ads), required activation e...

The absolute quantification of blood plasma metabolites by proton NMR spectroscopy is complicated by the presence of a baseline and broad resonances originating from serum macromolecules and lipoproteins. A method for spectral simplification of proton NMR spectra of blood plasma is presented. Serum macromolecules and metabolites are completely separated by utilizing the large difference in tran...