cryopreservation is as an effective way to prevent species extinction risk. in this study, we have investigated the protective effect of different concentrations cryoprotectant and effect of different dilution ratios on sperm motility percent and its motility duration after thaw. for this work dimethyl sulfoxide, methanol and ethylene glycol was used as cryoprotectant, each at concentration of ...

The aim of this study was to explore the effects of S-nitrosoglutathione (GSNO) on the aggregation of frozen platelets, the platelet nitric oxide (NO) content and the expression of membrane glycoproteins. The level of frozen platelet aggregation was measured using a platelet aggregation analyzer, while the content of NO was measured by the nitrate reductase method and the expression of frozen p...

The effect of sodium tetraborate (Na(2)B(4)O(7), borax) on the thermal property of frozen aqueous sugar and polyol solutions was studied through thermal analysis. Addition of borax raised the thermal transition temperature (glass transition temperature of maximally freeze-concentrated solutes; T(g)') of frozen sucrose solutions depending on the borax/sucrose concentration ratios. Changes in the...

The integrity of sperm progesterone (P4) receptor(s) and its response to steroid stimulation might be crucial for the maintenance of sperm fertilizing ability after cryopreservation. The aim of the current investigation was to study the effect of cryo-procedures on canine sperm P4 receptor(s). In addition, alteration of P4 receptor(s) at the molecular level and their functional integrity follow...

SUMMER 2008 BETA 31 Trauma : “I can’t remember anything after the first few words she said: ‘I’m sorry to tell you that your test results came back positive.’ After that, it was all just a blur of sounds and images—the traffic outside, my heart beating, the look on her face. Everything was moving in slow motion. I just kept hearing those five words echoing in my head: ‘Your test came back posit...

Various methods of cryopreservation of blood, especially erythrocytes, are generally known and have been used for a long time. Storage of blood in the frozen state presented one of the alternative ways of storing blood components; this possibility was intensively explored in the 1950’s and 60’s, when the shelf life of non-frozen red blood cells did not exceed 21 days at those times. This time l...

Protein S deficiency patient is characterized by recurrent thrombosis, and its risk is higher intraoperatively, especially in cardiac surgery involving cardiopulmonary bypass. Two heparin cessation periods are defined in cardiac surgery. One is the period between the cessation of heparin 4 to 5 h before surgery and the heparinization prior to cardiopulmonary bypass. The other is the period betw...

Grooves on the surface of the plasmalemma(plasma membrane) of frozen-etched yeast cells have been shown by many investigators (1, 10, 12) . Necas et al. (13) showed interconnected grooves on the surface of frozen-etched Saccharomyces cerevisiae protoplasts, but such structures could not be so effectively demonstrated in frozen-etched protoplasts prefixed in glutaraldehyde . Structures comparabl...

Fresh, frozen, and canned vegetables all have their own unique set of benefits and drawbacks, but all contain vital nutrients, such as carbohydrates, protein, vitamins, minerals, and fiber, which are maintained through the respective processes. Canned and frozen versions are the most common processed vegetables. While processed vegetables can be more convenient, have a longer shelf-life, and ar...

Fresh, frozen, and canned vegetables all have their own unique set of benefits and drawbacks, but all contain vital nutrients, such as carbohydrates, protein, vitamins, minerals, and fiber, which are maintained through the respective processes. Canned and frozen versions are the most common processed vegetables. While processed vegetables can be more convenient, have a longer shelf-life, and ar...