Fourier transform infrared (FTIR) spectroscopy is a mature analytical technique employed to examine polymeric materials. With the coupling of an infrared interferometer to a microscope equipped with specialized detectors, FTIR spectroscopy has been employed widely to examine microscopic areas in polymers for the last twenty years. Following the emergence of instrumentation techniques that emplo...

A rapid method for quantitative analysis of free fatty acids in virgin coconut oil (VCO) was determined using Fourier transform infrared (FTIR) spectroscopy. Thirty training samples of VCO were prepared by spiking lauric acid to VCO samples covering a range of 0.1% to 5% of free fatty acids. FTIR spectra between 1730 and 1690 cm-1 was used for quantitative determination of free fatty acids. A p...

The aim of this study was to compare polymerization lamps of various technologies (halogen, high performance halogen, xenon, i.e. plasma arc, as well as diode lamps) to determine the degree of polymerization of composite probes using Fourier-transform infrared (FTIR) spectroscopy. To observe the light-induced curing reaction under a bracket, an attenuated total reflection (ATR)-FTIR was used. T...

Fourier transform infrared spectroscopy (FTIR) difference spectroscopy in combination with deuterium exchange experiments has been used to study the photo-oxidation of P740, the primary electron donor in photosystem I from Acaryochloris marina. Comparison of (P740(+)-P740) and (P700(+)-P700) FTIR difference spectra show that P700 and P740 share many structural similarities. However, there are s...

Oxidation of fats and oils is an important indicator for performance and shelf life of oils. As other vegetable oils, virgin coconut oil (VCO) is not susceptible to lipid oxidation. Thus, a rapid method for quantitative analysis of peroxide value in VCO was developed in this study using Fourier transform infrared (FTIR) spectroscopy coupled with attenuated total reflectance (ATR). Thirty traini...

There is a requirement for a noninvasive technique to monitor stem cell differentiation. Several candidates based on optical spectroscopy are discussed in this review: Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, and coherent anti-Stokes Raman scattering (CARS) microscopy. These techniques are briefly described, and the ability of each to distinguish undifferentiated from...

Staphylococcus aureus capsular polysaccharides (CP) are important virulence factors and represent putative targets for vaccine development. Therefore, the purpose of this study was to develop a high-throughput method to identify and discriminate the clinically important S. aureus capsular serotypes 5, 8, and NT (nontypeable). A comprehensive set of clinical isolates derived from different origi...

Fourier transform infrared (FTIR) spectroscopy was used to study protein-cofactor interactions in photosystem II. The FTIR spectrum of each cofactor was measured as a difference spectrum upon its light-induced reaction. The spectrum included the bands of the protein moiety coupled to the cofactor, which were identified using selective isotopic labeling of amino acids. Analysis of these FTIR ban...

Three azo group-containing Schiff base ligands, namely 1-{3-[(3-hydroxypropylimino) methyl]-4-hydroxyphenylazo}-4-nitrobenzene (2a), 1-{3-[(3-hydroxypropylimino) methyl]-4-hydroxyphenylazo}-2-chloro-4-nitrobenzene (2b) and 1-{3-[(3-hydroxypropylimino) methyl]-4-hydroxyphenylazo}-4-chloro-3-nitrobenzene (2c) were prepared. The ligands were characterized by elemental analysis, FTIR spectroscopy, ...