From the INSERM, Centre d’Investigation Clinique CIC-P 9501, Nancy, F-54000 France (RE, RF, FZ, PR) ; CHU Nancy, Department of Cardiology, Nancy, F-54000 France (FZ) ; Université de Lorraine, Nancy, F-54000 France (FZ, PR) ; INSERM, U1116, Nancy, F54000 France (FZ, PR) ; Clermont Université, Université d’Auvergne, UMR6284, F-63000 Clermont-Ferrand France (RE) ; CHU Clermont-Ferrand, Department ...

We have raised antisera against human prothrombin activation fragment Fa in rabbits and have chromatographed the respective immunoglobulin G fractions on prothrombin-Sepharose, Prl-Sepharose, and FZ-Sepharose immunoadsorbents. The specific antibody population obtained was utilized to construct a double antibody radioimmunoassay capable of measuring as little as 0.8 rig/ml of this component. Our...

Background and Aim: Auditory P300 is an event-related potential. Cognitive factors like attention are involved in the generation of P300. It seems that normative variation of P300 is necessary for clinical purposes. Thus, the current study was designed to establish preliminary normative variation of P300 amplitude and latency at Fz and Cz sites in adults. Methods: This cross-sectional study was...

The frizzled (fz) gene in Drosophila controls two distinct signaling pathways: it directs the planar cell polarization (PCP) of epithelia and it regulates cell fate decisions through Armadillo (Arm) by acting as a receptor for the Wnt protein Wingless (Wg). With the exception of dishevelled (dsh), the genes functioning in these two pathways are distinct. We have taken a genetic approach, based ...

The Frizzled (Fz; called here Fz1) and Fz2 receptors have distinct signaling specificities activating either the canonical Wnt/beta-catenin pathway or Fz/planar cell polarity (PCP) signaling in Drosophila. The regulation of signaling specificity remains largely obscure. We show that Fz1 and Fz2 have different subcellular localizations in imaginal disc epithelia, with Fz1 localizing preferential...

The Drosophila frizzled (fz) gene is required for the development of normal tissue polarity in the epidermis. Genetic epistasis experiments argue that fz is at the top of a regulatory hierarchy that controls the subcellular site for prehair initiation within the cells of the pupal wing (Wong and Adler, 1993; J. Cell Biol. 123, 209-221). Genetic mosaic experiments indicate that fz has both cell ...

Acquisition of planar cell polarity (PCP) in epithelia involves intercellular communication, during which cells align their polarity with that of their neighbors. The transmembrane proteins Frizzled (Fz) and Van Gogh (Vang) are essential components of the intercellular communication mechanism, as loss of either strongly perturbs the polarity of neighboring cells. How Fz and Vang communicate pol...

Endosomal trafficking of signaling proteins plays an essential role in cellular homeostasis. The seven-pass transmembrane protein Frizzled (Fz) is a critical component of Wnt signaling. Although Wnt signaling is proposed to be regulated by endosomal trafficking of Fz, the molecular events that enable this regulation are not completely understood. Here we show that the endosomal protein Myopic (...