Antisera prepared against each of three single and one pair of major structural proteins of the bovine coronavirus (Mebus strain) were used in immunoblotting studies to measure cross-reactivity with the structural proteins of the human coronavirus OC43 and the mouse hepatitis coronavirus A59. We conclude that the bovine coronavirus is comprised of four major structural proteins, gp190 (normally...

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acute respiratory syndrome. New Engl. J. Med. 348, 1953–1966 3 Drosten, C. et al. (2003) Identification of a novel coronavirus in patients with severe acute respiratory syndrome. New Engl. J. Med. 348, 1967–1976 4 Peiris, J.S.M. et al. (2003) Coronavirus as a possible cause of severe acute respiratory syndrome. Lancet 361, 1319–1325 5 Ruan, Y.J. et al. (2003) Comparative full-length genome sequ...

The drastic increase in the number of coronaviruses discovered and coronavirus genomes being sequenced have given us an unprecedented opportunity to perform genomics and bioinformatics analysis on this family of viruses. Coronaviruses possess the largest genomes (26.4 to 31.7 kb) among all known RNA viruses, with G + C contents varying from 32% to 43%. Variable numbers of small ORFs are present...

Among different coronavirus genera, the receptor-binding S1 subunits of their spike proteins differ in primary, secondary, and tertiary structures. This study identified shared structural topologies (connectivity of secondary structural elements) in S1 domains of different coronavirus genera. The results suggest that coronavirus S1 subunits share a common evolutionary origin but have attained d...

The key enzyme in coronavirus replicase polyprotein processing is the coronavirus main protease, 3CL(pro). The substrate specificities of five coronavirus main proteases, including the prototypic enzymes from the coronavirus groups I, II and III, were characterized. Recombinant main proteases of human coronavirus (HCoV), transmissible gastroenteritis virus (TGEV), feline infectious peritonitis ...

the aim of the present study was to evaluate the presence anti- e. coli, -rotavirus and -coronavirus, in calves' sera. a total of 184 calves under 1 month of age (84 diarrheic & 100 normal) was studied. serological tests including: direct elisa for detection of anti-k99 e. coli, - rotavirus and - coronavirus and tube agglutination test for detection anti-o157 e. coli, antibodies were used....

background: middle east respiratory syndrome (mers) is a viral respiratory illness caused by a coronavirus. after the primary onset of mers in saudi arabia, in september 2015 cases began to increase. the number of laboratory-affirmed cases by mers-cov in the middle east has been being increased recently. method: in this current review article, by using the terms “mers” and “coronavirus” we firs...

In several mammalian species, including humans, coronavirus infection can modulate the host immune response. We show a potential role of dendritic cells (DC) in murine coronavirus-induced immune modulation and pathogenesis by demonstrating that the JAW SII DC line and primary DC from BALB/c mice and p/p mice with reduced expression of the murine coronavirus receptor, murine CEACAM1a, are suscep...

This is the first report of the production of monoclonal antibodies against elk coronavirus. The nucleoprotein gene of elk coronavirus was amplified by PCR and was cloned and expressed in a prokaryotic expression vector. Recombinant nucleocapsid protein was used to immunize mice for the production of hybridomas. Twelve hybridomas that produced monoclonal antibodies against the nucleocapsid prot...