Arylsulfatase A (cerebroside sulfate sulfohydrolase) was purified 3500-fold at a 7% yield from human urine. A crude urinary protein concentrate was prepared by treating pooled urine with ammonium sulfate and subsequently drying the precipitate with acetone. The powder thus obtained was extracted with buffer and was subjected to chromatographic and electrophoretic procedures as follows: (a) ammo...

The lectin of Limulus polyphemus haemolymph agglutinates erythrocytes [l-4] and other cells [ 51. Neuraminic acid has been shown to be the specific ligand of this lectin. The lectin has been isolated by ultracentrifugation and preparative starch gel electrophoresis [6], by DEAE-Sephadex and gel filtration chromatography [7] and by ultracentrifugation, affinity and gel filtration chromatography ...

In this research, the characteristics of CMP dioxane lignin (hardwood origin) and soda bagasse lignin (ethanol soluble fraction) and dioxane lignin of bagasse were studied using gel permeation chromatography, gas chromatography-mass spectrometry and 13C NMR spectroscopy and DFRC degradation procedure. The results indicated that the main structural units in lignins are phenyl propane skeletons b...

the edible mushrooms (basidomycetes) have high nutritional value, promote the immune system, and as a source of natural antimicrobial substances have been used to cure bacterial infections since ancient times.various kinds of proteins with several biological activities are produced by mushrooms. in this research, in order to evaluate antibacterial activity of edible mushrooms, we isolated prote...