The significance of endotoxin-free, sterile dialysis fluid for long-term, high-quality hemodialysis treatment is obvious and highly desired[MF1] . current study aimed to determine endotoxin the water quality used in twenty centers. Eighty samples (40 x dialysates 40 water) were tested using LAL gel clot method. results revealed a variation produced that makes it unaccepted compared internationa...

Porcine tissue-type plasminogen activator (t-PA) increases the binding of 125I-glu-plasminogen to clots made from human plasma or purified fibrinogen in a time and t-PA concentration dependent fashion. The accumulation of plasminogen was faster and greater on noncrosslinked plasma clots than on clots which had been crosslinked by Factor XIIIa. Furthermore, the uptake of plasminogen to crosslink...

ined hemolysis (H)-indices before and after implementing the AC mode on a large number of samples. H-indices were determined photometrically at 405 and 700 nm (5 ). Retrospective examination of H-indices from 42 124 samples processed in the CC mode was compared with H-indices from 30 483 samples processed in the AC mode. The distributions of the different grades of hemolysis were identical for ...

Neutrophil extracellular traps are networks of DNA and associated proteins produced by nucleosome release from activated neutrophils in response to infection stimuli and have recently been identified as key mediators between innate immunity, inflammation, and hemostasis. The interaction of DNA and histones with a number of hemostatic factors has been shown to promote clotting and is associated ...

We have studied the contribution of platelets to the coagulation of plasma and the effects of activation or inhibition of platelets on the coagulation process in unanticoagulated fresh whole blood (subsequently termed native blood). For this purpose, we have used a free oscillation rheometer (FOR), the ReoRox4, a new instrument that enables noninvasive viscoelastic measurements of clot formatio...

Two previously undescribed activators of prekallikrein have been purified from human plasma by alcohol frac tionation, ion exchange chromatography, gel filtration, and polyacrylamide gel electrophoresis. Both were apparently homogeneous as judged by electrophoretic mobility and molecular weight. A monomer-dimer relationship between these two activators is suggested by the use of disc gel electr...

Autologous blood transfusion is the safest and most successful way to decrease transfusion-related risks such as postoperative infections, allo-immunization, and short- and long-term immunosuppression. In addition, these fibrin sealants are known to provide coagulation support at the surgical site and act as an adjunct to the control of postoperative bleeding. The physical formation of autologo...

Crude or crystalline trypsin in proper concentration causes the blood or plasma of human beings, dogs, rabbits, guinea pigs, and horses to coagulate. It does not clot the fibrinogen directly, but reacts with prothrombin to form thrombin. Since trypsin thus has the same effect as the physiological system Ca plus platelets (or Ca plus tissue extracts), it is suggested as a tentative working hypot...

We have previously demonstrated that increasing factor XIII concentrations above that present in plasma (1 U/mI) results in the formation of very high molecular weight a fate polyacrylamide and agarose gel electrophoresis (SDSPAGE). In this report. we have examined the effect of such crosslinking on plasmic susceptibility of fibrin prepared from purified fibrinogen and from plasma in the presen...