نتایج جستجو برای: gel filtration chromatography
تعداد نتایج: 226456 فیلتر نتایج به سال:
A high-affinity riboflavin -binding protein was isolated and characterized for the first time from pregnant-rat sera by affinity chromatography on a lumiflavin-agarose column. The purified protein was homogeneous by the criteria of analytical polyacrylamide-gel disc electrophoresis, gel-filtration chromatography on Sephadex G-100 and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. I...
Supplement 1: Methods Purification procedure of tissue kallikrein Rat kallikrein was purified from urine to apparent homogeneity by ultrafiltration and ion-exchange chromatography on a DEAE-Sepharose FF column (GE Healthcare, Buckinghamshire, UK), affinity chromatography on an aprotinin (Sigma, MO, USA) coupled HiTrap NHS-activated Sepharose HP column (GE Healthcare) and gel filtration twice on...
Abstract Background: Rabies is the most important zoonosis in country. Anti-viral antibody conjugated with Fluorescein Isothiocyanate (FITC), is used for diagnosis of Rabies. We import this reagent from abroad. In this study we aimed to prepare this conjugated fluorescein antibody and assess its utility. Materials and methods: Rabbits were immunized with rabies vaccine, then serum samples ...
An aminopeptidase activity capable of hydrolyzing different aminomethylcoumarin amino acids, but mainly leucine-7-amino-4-methylcoumarin (Leu-NHMc), was detected in deoxycholic soluble extracts from adult Fasciola hepatica. The enzyme (EC 3.4.11.1) was partially purified by gel filtration and EAH-Sepharose affinity chromatography using bestatin as a ligand. Results obtained by gel filtration, d...
Urea amidolyase was purified to homogeneity from extracts of Candida utilis. The purification involves protamine sulfate precipitation, ammonium sulfate precipitation, polyethylene glycol precipitation, Sepharose 6B gel filtration, DEAE-cellulose column chromatography, and hydroxylapatite column chromatography. The final preparation is pure as judged by disc-gel electrophoresis. The molecular w...
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