the present study deals with development of a floating in-situ gel of the narrow absorption window drug baclofen. sodium alginate-based in-situ gelling systems were prepared by dissolving various concentrations of sodium alginate in deionized water, to which varying concentrations of drug and calcium bicarbonate were added. fourier transform infrared spectroscopy (ftir) and differential scannin...

The protocol consists of running a native gel with in-gel digestion by proteases, subsequent mass spectrometrical determination of protein sequence and modifications, followed by electro-elution and conformational analysis using melting point and circular dichroism. Finally, the eluted protein is tested for preserved function. Herein, C1 esterase inhibitor is applied on a native gel; ingel dige...

The effect of colloidal silica fillers on the cross-linking behavior of a model UV-curable polymer system (thiol-ene) is studied using in situ rheology and real-time FTIR spectroscopy. The validity of the Winter-Chambon criterion (convergence of the loss tangents at the gel point) is examined for the cross-linking of these filled polymers, some of which are strongly flocculated dispersions (phy...

OBJECTIVES The purpose of this study was to determine the hospital-wide incidence of bacterial contamination of point-of-care (bedside) ultrasound probes and coupling gel at a single academic medical center to predict the risk of nosocomial spread of infection. METHODS Bacterial cultures were performed on all point-of-care ultrasound probe surfaces and associated gel bottles in our institutio...

BACKGROUND Real-time polymerase chain reaction (PCR) is generally regarded as a very accurate and time-saving method, but it is expensive to run. We evaluated the reliability of an inexpensive and a researcher-friendly gel electrophoresis-based PCR method for the quantification of mRNA, and the results were compared with those obtained by real-time PCR. METHODS We compared the results of rela...

The concentration dependence of dynamic moduli G’ and G” of soy protein isolates (SPI) and soy protein concentrates (SPC) were studied in solutions prepared under different conditions. Three-level preliminary fractional experiments were undertaken to study effects of temperature, pH, concentration on the gel strength of soy protein isolate and soy protein concentrate solutions. Protein concentr...

The present study deals with development of a floating in-situ gel of the narrow absorption window drug baclofen. Sodium alginate-based in-situ gelling systems were prepared by dissolving various concentrations of sodium alginate in deionized water, to which varying concentrations of drug and calcium bicarbonate were added. Fourier transform infrared spectroscopy (FTIR) and differential scannin...

The present study deals with development of a floating in-situ gel of the narrow absorption window drug baclofen. Sodium alginate-based in-situ gelling systems were prepared by dissolving various concentrations of sodium alginate in deionized water, to which varying concentrations of drug and calcium bicarbonate were added. Fourier transform infrared spectroscopy (FTIR) and differential scannin...

It has been determined that the bag cells of Aplysia californica produce two polypeptide species that comigrate on electrophoretic gels containing sodium dodecyl sulfate. By this separation procedure both species can be assigned a molecular weight of approximately 6,000. One of these molecules has an Rf of 0.65 on alkaline discontinuous electrophoresis gels, an isoelectric point at pH 4.8, a ge...