background: the interaction between follicular cells and oocyte leads to a change in gene expression involved in oocyte maturation processes. objective: the purpose of this study was to quantify the expression of more common genes involved in follicular growth and oocyte developmental competence. materials and methods: in this experimental study, the expression of genes was evaluated with qrt-p...

objective: rna interference (rnai) is the most potent technique for gene silencing in eukaryotic cellular system at transcriptomic level. genetic disorders and cancers are important targets for therapeutic development of this technique. in order to bypass the temporary dpwnregulation by sirna, a new generation of shrna named shrnamir has developed. silencing construct with structure similar to ...

suicide is a major public health concern, with approximately one million people committing suicide world-wide each year .risk factors for suicidal behavior include, psychiatric and medical illness, impulsivity, aggression, alcohol and drug abuse specially stimulants, and  stress. neurobiological, brain mappings and biochemical studies have revealed that suicide victims experiencing a large numb...

background: apoptosis is a process that plays an important role during early stage of implantation. the aim of this study was to investigate the incidence of apoptosis in mice endometrium after ovarian stimulation at implantation period. methods: nmri female mice were divided into two groups: 1) control group, which were rendered pseudopregnant by vaginal stimulation and 2) experimental group, ...

schizophrenia (scz) is a complex disorder with no clarified etiology or biological diagnosis.  symptoms of schizophrenia including positive, negative and cognitive deficits.the neurodegenrative and neurodevelopmental hypothesis are main views  about the pathophysiology of schizophrenia. neuregulin 1 is one of four proteins in the neuregulin family that act on the egfr family of receptors. nrg1 ...

Background Follicle stimulating hormone (FSH)- a hetrodimeric glycoprotein- is secreted by pituitary gland. This hormone stimulates growth and maturation of the follicles in females and sperms in male. Up to now, glycoprotein hormones such as FSH have produced in different cell lines. Among of the mammalian expression systems, the Chinese hamster ovary cells (CHO) have taken into consideration ...

background: parathyroid hormone is an 84-amino acid peptide secreted by the parathyroid glands. its physiological role is maintenance of normal serum calcium level and bone remodeling. biological activity of this hormone is related to n-terminal 1-34 amino acids. the recombinant form of hormone (1-34) has been approved for treatment of osteoporosis from 2002. in this study, a novel fusion partn...

objective(s): streptococcus pyogenes produces extracellular hyaluronidase enzyme. this enzyme is directly associated with the spread of the organism during infection. the objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity. materials and methods: the enzymatic region of hyaluronidase gene was detecte...