DNA fabrication of genetic cassettes at base-level precision is transforming genetic engineering from a laborious art to an information-driven discipline. Although substantial advances have been made in the development of DNA fabrication, the methods employed vary widely based on the length of the DNA. All of these methods are available commercially, but can also be performed at the molecular b...

Gene expression programming presents an alternative approach in the evolutionary computation paradigm evolving populations of candidate solutions as valid computer programs that can be used for a potentially wide range of problem solving tasks, including sound synthesis. This paper proposes Gene Expression Synthesis (GES) as a method to evolve sound synthesis functions as nested graphs of unit ...

With gene synthesis we will in future be able to synthesize complete genomes. From there, we will be able to answer essential biological questions, for example, how chemical reactions proceed within a cell. The first step, genome sequencing, especially of the human genome, has created a huge amount of data. It is currently estimated that humans have about 25000 genes. Chemically speaking, genes...

To test the hypotheses from genomics, synthetic biology, and bioinformatics, accurate and cost-effective gene and genome synthesis is necessary. Here, a microchip-based technology is proposed for integrating multiple functions required in gene synthesis. To date, there is no report of high-throughput gene synthesis with high-level of integration. This approach would be a significant step toward...

De novo synthesis of long double-stranded DNA constructs has a myriad of applications in biology and biological engineering. However, its widespread adoption has been hindered by high costs. Cost can be significantly reduced by using oligonucleotides synthesized on high-density DNA chips. However, most methods for using off-chip DNA for gene synthesis have failed to scale due to the high error ...

background: asthma is very common in children and its diagnosis is based on clinical manifestations, which can be misdiagnosed as other respiratory diseases with similar signs and symptoms. objective: to analyze the expression of st2l and cd203c in the diagnosis of pediatric asthma. methods: basophils were purified from whole blood samples of patients and healthy controls using ficol-paque grad...

Phenolics in the Chinese olive (Canarium album (Lour.) Raeusch) fruit significantly affect its flavor and quality. The shikimate pathway is a bridge connecting primary metabolism secondary through which fixed carbon can be transformed into phenolics. In this study, we aimed to reveal relationship between phenolic compound biosynthesis. Three fruits (cv. Tanxiang (TX), Changying (CY) Lingfeng (L...

Objective(s) Nonviral vector can be an attractive alternative to gene delivery in experimental study. In spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. To determine the effective expression, the recombinant Escherichia coli lacZ genes were cloned into the different variants of pcDNA3.1 and the...