BACKGROUND/AIMS Genipin, the aglycon of geniposide found in gardenia fruit has long been considered for treatment of various diseases in traditional oriental medicine. Genipin has been used as a blue colorant in food industry. Genipin has recently been reported to have some pharmacological functions, such as antimicrobial, antitumor, and anti-inflammatory effects. The aim of this study was to e...

An efficient cold-mechanical/sonic-assisted extraction technique was developed for extraction of genipin from genipap (Genipa americana) peel. Ultrasound assisted extraction (285 W, 24 kHz) was performed at 5, 10 and 15 °C for 5, 10 and 15 min. After cold-extraction, genipin was separated from pectin and proteins by aid of fungal pectinesterase. The maximum yield of non-cross-linked genipin was...

Our study is about modifying three-dimensional (3D) collagen hydrogels with a genipin compound. Specifically, the study set out to answer a question if genipin crosslinking modifies the nanoand microstructure of collagen and to establish for the first time a combination of methods to follow this process in-situ and in real time. We examined the genipin cross-linked collagen hydrogels with one-p...

Uncoupling protein-2 (UCP2) is known to suppress mitochondrial reactive oxygen species (ROS) production and is employed by drug-resistant cancer cells to mitigate oxidative stress. Using the drug-sensitive HL-60 cells and the drug-resistant MX2 subline as model systems, we show that genipin, a UCP2 inhibitor, sensitizes drug-resistant cells to cytotoxic agents. Increased MX2 cell death was obse...

Genipin-crosslinked chitosan hydrogels have gained attention as promising drug delivery vehicles. To bring them one step closer to clinical applications, this study evaluates the hydrogels' in vitro biocompatibility and biodegradation. Cytotoxicity test on 3T3 fibroblasts shows that cells retain normal adhesive properties high viability gels with 3.1 4.4 mm genipin but not 1.7 genipin. The high...

This research aims to develop an alternative method to osteochondral articular transfer using decellularized, crosslinked porcine osteochondral xenografts (OCXGs). Crosslinking collagenous tissue results in greater mechanical strength, more resistance to enzymatic degradation, and reduced immunogenicity. This study used genipin, a chemical substance from the Gardenia jasminoides Ellis fruit. Ge...

Genipin, an iridoid compound extracted from gardenia fruits (Gardenia jasminoides Ellis) is a natural crosslinking agent. Because of its low cytotoxicity, genipin is increasingly used to replace both glutaraldehyde and formaldehyde as a crosslinker for proteins. Researchers have in recent years successfully demonstrated the effective use of genipin in the modification of gelatin, particularly f...

INTRODUCTION: The feasibility of using crosslinking reagent, e.g., the genipin or methylglyoxal, to enhance the biomechanical functions of the injured or degenerated intervertebral disc has been extensively investigated. Our previous study found that genipin could recover the disc dynamic properties of mild denatured disc; however, the recovery efficiency was degraded by fatigue loading. Genipi...

Bone marrow-derived mesenchymal stem cells (BMSCs) have shown great promise for ischemic tissue repair. However, poor viability of transplanted BMSCs within ischemic tissues has limited their therapeutic potential. Numerous evidences suggested that reactive oxygen species (ROS) generated and apoptosis play an important role in regulation BMSCs loss at the ischemic site. Uncoupling protein 2 (UC...