This protocol describes a series of procedures for isolating nucleic acids (DNA and RNA) and proteins from moss (Physcomitrella patens) tissue. This series includes a rapid, small-scale procedure for isolating DNA, which results in genomic DNA that is only suitable for polymerase chain reaction (PCR), as well as a method for obtaining much larger amounts of genomic DNA suitable for Southern ana...

With the rapid development of molecular biology and the life sciences, magnetic extraction is a simple, automatic, and highly efficient method for separating biological molecules, performing immunoassays, and other applications. Human blood is an ideal source of human genomic DNA. Extracting genomic DNA by traditional methods is time-consuming, and phenol and chloroform are toxic reagents that ...

Colorectal cancer (CRC) is one of the most common forms of cancers in the world and is curable if diagnosed at the early stage. Analysis of DNA extracted from stool specimens is a recent advantage to cancer diagnostics. Many protocols have been recommended for DNA extraction from stool, and almost allof them are difficult and time consuming, dealing with high amount of toxic materials like phen...

The aim of the present study was to develop a simple, rapid and inexpensive protocol of ultrapure bacterial genomic DNA extraction suitable for use in molecular methodology. This communication describes a protocol which uses superparamagnetic bare nanoparticles for isolation and purification of genomic DNA from overnight culture of Escherichia coli XL1 Blue strain. A comparison is also made wit...

colorectal cancer (crc) is one of the most common forms of cancers in the world and is curable if diagnosed at the early stage. analysis of dna extracted from stool specimens is a recent advantage to cancer diagnostics. many protocols have been recommended for dna extraction from stool, and almost allof them are difficult and time consuming, dealing with high amount of toxic materials like phen...

background: soybean oil is one of the highly consumed vegetable oil worldwide. nowadays, usage of genetically modified (gm) soybean seeds for soybean oil production is constantly increasing. the recommended methods for gmo detection are based on analysis of residual dna in vegetable oil and highly processed food. however, the successful amplification of isolated dna depends on the efficiency of...

Effective microchip extraction of deoxyribonucleic acid (DNA) from crude biological matrixes has been demonstrated using silica beads or hybrid phases composed of beads and sol-gel. However, the use of monolithic sol-gels alone for extraction of human genomic DNA has been more difficult to define. Here we describe, for the first time, the successful use of monolithic tetramethyl orthosilicate-b...

We present a strategy to recover high molecular weight genomic DNA from large numbers of isolates of Phytophthora. Included are steps for generating mycelial mass in 24-well reuseable deep well plates, efficient lyophilization and disruption of the mycelium and genomic DNA extraction with 96-well glass fiber filter plates. The resulting DNA is consistently high molecular weight and is suitable ...