background: classification of molar gestation into complete hydatidiform mole (chm) and partial hydatidiform mole (phm) is done according to clinical, ultrasonographic, histologic and genetic criteria. however, making a distinction between chm and phm using histologic criteria alone may be difficult and several studies have shown that misclassifications are frequent, even for experienced pathol...

objectives: this current research was conducted in order to analyze the molecular characterization of isolated cryptosporidium spp. in the southwest of iran. materials and methods: in this survey, 390 fecal samples were collected from immunocompromised individuals and children under five-years-of-age. parasitic infection was evaluated using wet mount preparation, formalin ether, a modified acid...

methods and materials: hepatitis b virus dna was detected by conventional pcr in some of the serum samples obtained from hbsag-positive chronic hepatitis b (chb) patients who were referred to health centers of shahrekord for routine monitoring of the disease. subsequently, using real-time pcr, the dna samples were used for genotyping and analysis of resistance to lamivudine. results: the dna wa...

background and aims: rubella virus is a human pathogen that causes congenital rubella syndrome (crs) when infection occurs during early pregnancy. vaccination programs have been remarkably successful in controlling natural rubella infection and crs. moreover, ongoing surveillance for all cases of rubella and crs is a vital component of a prevention program. although the who recommends the use o...

abstract snp typing is now a well-established genotyping system in bacillus anthracis studies. in the original standard method of van erth, snps at 13 loci of the b. anthracis genome were analyzed. in order to simplify and make appropriate this expensive method to low-budget laboratory settings, 13 primer pairs targeting the 13 corresponding snps were designed. besides, a universal pcr protocol...

1320 blood samples were collected from herds showing clinical signs of pestiviral infections. 39 samples were positive in ag-elisa assay. all these 39 samples in addition to 5 cytopathic strains were cultured in md-bk cell line and presence of pestiviral antigens was confirmed by direct-immunofluorescent test. 27 out of 44 bvdv suspected isolates were detected by rt-pcr using a primer set. diff...

background and aims: hepatitis b virus (hbv) infects the liver and causes acute and chronic hepatitis, hepatocellular carcinoma and cirrhosis. hbv has been divided to eight genotypes (a–h) and subgenotypes of a, b, c and f. for the first time, we determined hbv genotypes in infected samples by inno-lipa method in yazd, central province of iran. materials and methods: this study was performed on...

background hepatitis c virus (hcv) is the major cause of chronic liver disease. hcv is a single stranded positive sense rna of approximately 9.6 kb. because of high conservativeness of 5΄untranslated region of hcv genome, it is widely used for virus genotyping. different methods are used for the virus genotyping, but all involve some difficulties. objectives the aim of the present study was to ...