A comprehensive effort was devoted to exploring, collecting and characterizing the local Maltese olive germplasm, often represented by ancient, monumental trees plants of uncertain origin. SSR cp-SSR analysis all samples enabled identification 46 genotypes establishment correspondence between ancient trees, main varieties other Mediterranean cultivars. The application plastid markers two lineag...

The genus Echinacea, Asteraceae, is comprised of nine species, which are perennial herbs indigenous to North America and which have been traditionally used as medicinal plants for centuries. Three Echinacea species, E. angustifolia DC var. angustifolia, E. purpurea (L.) Moench, and E. pallida (Nutt.) Nutt., are currently being traded internationally in the natural products market. Echinacea pro...

Peach (Prunus persica (L.) Batsch) is one of the most produced and studied stone fruits. Many genetic genomic resources are available for this species, including a high-quality genome. More recently, new high-density Illumina peach Single Nucleotide Polymorphism (SNP) chip (9+9K) has been developed by an international consortium as add-on to previous 9K array. In current study, array was used s...

Evaluation of the genetic diversity among populations is an essential prerequisite for the preservation of endangered species. Thousands of new accessions are introduced into germplasm institutes each year, thereby necessitating assessment of their molecular diversity before elimination of the redundant genotypes. Of the protocols that facilitate the assessment of molecular diversity, SSRPs (si...

Creating transgenic plants is invaluable for the genetic analysis of sugar beet and will be increasingly important as sugar beet genomic technologies progress. A protocol for Agrobacterium-mediated transformation of sugar beet is described in this chapter. Our protocol is optimized for a sugar beet genotype that performs exceptionally well in tissue culture, including the steps of dedifferentia...

Plant cryopreservation is useful for long term storage of clonal germplasm and endangered species. Clonally propagated crops which produce recalcitrant seeds cannot be easily conserved using conventional methods. Preservation plants in vitro limited to two years not ideal a very time. The need conserve plant genetic resources through techniques mitigate the effects climate change such as extinc...

The incidence of clavicipitaceous anamorphic endophytes in a Hordeum spp. germplasm collection is reported. The potential application of endophytes as biocontrol agents against pests of cereal crops is recognized. Suggestions are proposed to modify existing germplasm maintenance procedures to ensure that both seed viability and endophyte viability and diversity are maintained in grass ,germplas...

For more than 25 years, systematic gathering and cryo-storage of biomaterials from diverse wild species have been ongoing to save gene diversity and improve captive (ex situ) and wild (in situ) animal management. Cryo-storage of biomaterials offers broad opportunities from helping understand the fundamental biology of unstudied species to enhanced conservation breeding, genomics and veterinary ...