Light-sheet microscopy enables fast 3D, high-contrast imaging in biology and colloidal sciences. Recently, the controlled transport of living embryos or small colloids through stable glass capillaries is manifold interesting. Although they hardly impair the sample, glass capillaries spoil the image by generating significant aberrations of the illumination and detection light. Here, we analyze t...

Glass microelectrodes (also called pipettes) have been a workhorse of electrophysiology for decades. Today, such pipettes are made from glass capillaries using a programmable puller. Such instruments heat the capillary using either a metal filament or a laser and draw out the glass using gravity, a motor or both. Pipettes for patch-clamp recording are formed using only heat and gravity, while s...

To record extracellular nerve activity, wires or polyethylene suction electrodes are commonly used, which are modified according to particular experimental requirements. For electrophysiological studies in a dissected animal, a length of the nerve under investigation may be lifted on to silver wires (Pearon, Stein & Malhotra, 1972), or a fine wire hook (Wilkens & Wolf, 1974), often under minera...

A sequential injection capillary immunoassay system (SI-CI) was developed to automate the multi-step competitive bioassay procedures for hyaluronan (HA), a potential biomarker for liver and cancer diseases. Biotinylated HA-binding proteins (b-HABPs), anti-biotin conjugated with horseradish peroxidase (anti-b-HRP) and substrate 3,3',5,5' tetramethylbenzidene (TMB) were employed. An ordinary glas...

Soon after the glass micropipette was invented as a micro-tool for manipulation of single bacteria and the microinjection and microsurgery of living cells, it was seen to hold promise as a microelectrode to stimulate individual cells electrically and to study electrical potentials in them. Initial successes and accurate mechanistic explanations of the results were achieved in giant plant cells ...

Super-resolution virtual imaging by micron sized transparent beads (microspheres) was recently demonstrated by Wang et al. Practical applications in microscopy require control over the positioning of the microspheres. Here we present a method of positioning and controllable movement of a microsphere by using a fine glass micropipette. This allows sub-diffraction imaging at arbitrary points in t...

Abstract Acoustic trapping is a useful method for handling biological samples in microfluidic systems. The aim of this work twofold: first to investigate the physics behind acoustic glass capillary and secondly perform binary trapping. latter achieved by increasing density fluid channel. device consisted with rectangular inner cross-section (height 200 µ m × width 2000 m) equipped small piezoel...