An experiment was conducted to evaluate the effect of different levels of germinated barley (GB) on live performance and carcass traits in broiler chickens. The experiment lasted for 5 weeks starting from 7 days of age and ending at 42 days of age. Chicks (Ross 308) were fed six dietary treatments including a corn–soy diet (corn diet), a barley–soy diet (barley diet), a barley diet plus enzymes...

An enzyme that has both beta-1,4-glucanase and chitosanase activities was found in the culture medium of the soil bacterium Lysobacter sp. IB-9374, a high lysyl endopeptidase-producing strain. The enzyme was purified to homogeneity from the culture filtrate using five purification steps and designated Cel8A. The purified Cel8A had a molecular mass of 41 kDa, as estimated by sodium dodecyl sulfa...

A 971-bp cDNA, designated licA, was obtained from a library of Orpinomyces sp. strain PC-2 constructed in Escherichia coli. It had an open reading frame of 738 nucleotides encoding LicA (1,3-1,4-b-D-glucanase; lichenase) (EC 3.2.1.73) of 245 amino acids with a calculated molecular mass of 27,929 Da. The deduced amino acid sequence had high homology with bacterial b-glucanases, particularly in t...

three hundred and sixty broiler chicks (ross 308) were employed in an experiment from 6 to 49 days of age to evaluate the effect of replacing canola meal for soybean meal either with or without enzyme supplementation. dietary treatments included 5 substitution levels (0, 25, 50, 75 and 100%) at two enzyme concentrations of: 0 and 400 g/ton in a 5×2 factorial layout experiment. the enzyme mixtur...

Fungal cell wall degrading chitinases and glucanases attained significance in agriculture, medicine, and environment management. The present study was conducted to describe the optimum conditions required for the production of beta-1,4-N-acetyl glucosaminidase (NAGase) and beta-1,3-glucanase by a biocontrol strain of Bacillus subtilis AF 1. B. subtilis AF 1 was grown in minimal medium with coll...

The beta-glucanase gene (bgl) from Bacillus amyloliquefaciens was expressed in E. coli CSH 55 under the control of the PR promoter of phage lambda that is repressed by the thermosensitive repressor C1857. Production of beta-glucanase was drastically stimulated by a temperature shift to 42 degrees C. This overexpression of the bgl gene (about 20% of the total cellular protein) led to an almost c...

This study was performed with growing chicken (14 to 56 d of age) to evaluate the effects of feeding them a barley or triticale-based diet. The treatments were corn diet (1) as a control, hulless barley diet with (5) or without (2) enzyme, triticale diet with (6) or without (3) enzyme and hulled barley diet with (7) or without (4) a commercial β-glucanase enzyme. In a digestibility trial, 21 ma...

A total number of  5520 female and  480 male breeders (Arbor Acres plus) at 42wks of age  were used in a 10-week-trial to investigate the effect of an enzyme complex on  the quality  and quantity of egg production as well as hatchability of broiler breeders fed a corn-soybean meal diet. There were two dietary treatment groups: a control group fed on a corn-soybean based diet, and the multi-enzy...

Twelve isolates of Trichoderma spp. isolated from tobacco rhizosphere were evaluated for their ability to produce chitinase and β-1,3-glucanase extracellular hydrolytic enzymes. Isolates ThJt1 and TvHt2, out of 12 isolates, produced maximum activities of chitinase and β-1,3-glucanase, respectively. In vitro production of chitinase and β-1,3-glucanase by isolates ThJt1 and TvHt2 was tested under...

The study investigated the efficacy of two isolates of Aspergillus terreus (65P and 9F) on the growth, morphology and pathogenicity of Pythium aphanidermatum on cucumber. In vitro tests showed that the two isolates inhibited the growth of P. aphanidermatum in culture. Investigating P. aphanidermatum hyphae close to the inhibition zone showed that the hyphae showed abnormal growth and loss of in...