We reported previously that D-glucosamine or a close derivative was involved in the synthesis of the N-methyl-L-glucosamine moiety of streptomycin'). Since D-glucosamine is a component of the cell wall of streptomycetes2,3), this sugar or its derivatives may be a common precursor of streptomycin and the cell wall. We studied also whether UDP-N-methyl D glucosaminephosphate was involved in the s...

1. Glycopeptides containing mannose were extracted from isolated yeast cell walls by ethylenediamine and purified by treatment with Pronase and fractionation on a Sephadex column. 2. A glycopeptide that appeared homogeneous on electrophoresis and ultracentrifugation had a molecular weight of 76000, and contained a high-molecular-weight mannan and approx. 4% of amino acids. 3. The amino acid com...

In the course of testing the possible coenzymic function of the phosphorylated vitamin B, derivatives on the synthesis of glucosamine 6-phosphate by a rat liver preparation, the appearance of a yellow color similar to that of pyridoxal 5-phosphate was observed in the samples containing pyridoxamine 5-phosphate. Further investigation has revealed the presence of a soluble enzyme system in extrac...

Many medically important biofilm forming bacteria produce similar polysaccharide intercellular adhesins (PIA) consisting of partially de-N-acetylated β-(1 → 6)-N-acetylglucosamine polymers (dPNAG). In Escherichia coli, de-N-acetylation of the β-(1 → 6)-N-acetylglucosamine polymer (PNAG) is catalysed by the carbohydrate esterase family 4 deacetylase PgaB. The de-N-acetylation of PNAG is essentia...

The behavior of 2-ethoxy-(4H)-3,1-benzoxazin-4-one (1) towards nitrogen nucleophiles, e.g. ethanolamine, aromatic amines (namely: p-toluidine, p-anisidine, p-hydroxyaniline, o-hydroxyaniline, o-bromoaniline, o-phenylenediamine, p-phenylenediamine, o-tolidinediamine) p-aminobenzoic acid, glucosamine hydrochloride, 2-aminonicotinic acid, 1-naphthalenesulfonic acid hydrazide, n-decanoic acid hydra...

Muramic acid, glucosamine, mannosamine and galactosamine in soils may be useful for elucidating the microbial origin of soil organic nitrogen. Therefore, a method was developed to determine the aldononitrile acetate derivatives of the four amino sugars simultaneously in the 6 M HCl hydrolysates of soil samples by means of high resolution gas li uid chromatography. This method was sensitive enou...

background: use of glucosamine as an alternative treatment for osteoarthritis is becoming more frequent, including in those who have diabetes at the same time. the results from in vitro and animal studies propose that glucosamine may inversely affect glucose metabolism. however, the recommended dose of oral glucosamine in healthy people or diabetics did not have such effects consistently. the a...

Chitin (β-(1-4)-poly-N-acetyl-D-glucosamine) is widely distributed in nature and is the second most abundant polysaccharide after cellulose. It is often converted to its more deacetylated derivative, chitosan. Previously, many reports have indicated the accelerating effects of chitin, chitosan, and its derivatives on wound healing. More recently, chemically modified or nano-fibrous chitin and c...

In this study it could be shown that in rat the normally occurring N-acetyl neuraminic acid can be modified in its N-acyl moiety by in vivo administration of the chemically synthesized N-propanoyl precursors, N-propanoyl-D-glucosamine or N-propanoyl-D-mannosamine. It could be shown that each of these nonphysiological amino sugar analogues was incorporated into both membrane and serum glycoprote...