The alkaliphilic halotolerant bacterium Bacillus sp. N16-5 is often exposed to salt stress in its natural habitats. In this study, we used one-colour microarrays to investigate adaptive responses of Bacillus sp. N16-5 transcriptome to long-term growth at different salinity levels (0%, 2%, 8%, and 15% NaCl) and to a sudden salt increase from 0% to 8% NaCl. The common strategies used by bacteria ...

The effects of substance P (SP) and baclofen were studied in the isolated spinal cord of newborn rats. Potential changes generated in motoneurones were recorded extracellularly from the ventral root (L3-L5). When SP (8 X 10(-5) M) was introduced into the bath, the depolarization of motoneurones began with a delay of 1.1 s. A large part of this delay can be explained as a time needed for SP to r...

Cu (II) ions, which cause serious diseases such as cancer and liver damage, have an important place, especially in water pollution. To effectively remove these deadly copper ions from aqueous solution, the synthesis of a new functionalized sporopollenin microcapsules (Sp-CPTS-HNMAA) to be used adsorbent was aimed. Schiff base (HNMAA), functionalize surface sporopollenin, obtained result reactio...

A gene (aly) encoding alginate lyase (ALY; EC 4.2.2.3) was isolated from a library constructed with the cosmid vector pHC79 and Sau3AI-digested genomic DNA of Pseudomonas sp. OS-ALG-9. Successive subcloning of the aly-containing cosmid enabled us to locate the gene on a 2.3 kb HpaI fragment. Nucleotide sequencing of this fragment revealed a single open reading frame (ORF) of 1365 bp. The direct...

In this study, favorable carbon-nitrogen ratio for high yields of gibberellic acid (GA3) production from Pseudomonas sp. was investigated. First of all, optimum carbon (glucose, maltose, sucrose, fructose, lactose) and nitrogen (KNO3, NH4Cl, NaNO3, urea, glycine) sources among the others were chosen. The highest yield of GA3 productivity was found in growth medium supplemented with fructose (16...

The goal of this study was to correlate synaptic ultrastructure with transmitter specificity and function in the lateral superior olive (LSO), a nucleus that is thought to play a major role in sound localization. This was accomplished by means of postembedding immunogold immunocytochemistry. Four classes of synaptic terminals were identified in the LSO. They were distinguishable from one anothe...

Extensive evidence implicates Substance P [SP(1-11)] as a primary afferent neurotransmitter or modulator of nociceptive information, and there is increasing evidence that the excitatory amino acids aspartate (Asp) and glutamate (Glu) may also act as nociceptive neurotransmitters. We have previously demonstrated that nociceptive stimulation (metatarsal injection of formalin) caused a tetrodotoxi...

reaction of glycin with malononitrile afforded respective pyrrole. pyrrole is one of the heterocyclic compounds with very important biological activities. heterocycles are extremely important because of their wide range of applications that goes from their use as pharmaceuticals and agrochemicals to dyestuffs and additives. in this view, it was proposed to synthesize pyrrole-3-carbonitrile deri...

soybean, glycine max, is susceptible to a large number of disease agents such as seedling and root pathogens that cause serious damages to this crop plant. one of these soil-borne pathogens is macrophomina phaseolina, the causal agent of charcoal root rot. in this study, two bacteria, pantoea agglomerans and bacillus sp. bin, and a fungus, trichoderma harzianum t100, as potential biocontrol age...