The developmental course of activity changes of 4 endogenous membrane glycosyltransferases : UDP-glucose : glycosyltransferase, UDP-galactose : glycosyltransferase, GDP-mannose: glycosyltransferase, and UD-PiV-acetylglucosamine : glycosyltransferase was determined in various stages of developing sea-urchin embryos from unfertilized eggs up to the 16-cell stage. The results show that there is a ...

The developmental course of activity changes of 4 endogenous membrane glycosyltransferases: UDP-glucose : glycosyltrasferase, UDP-galactose : glycosyltransferase, GDP-mannose: glycosyltransferase, and UD-PN-acetylglucosamine : glycosyltransferase was determined in various stages of developing sea-urchin embryos from unfertilized eggs up to the 16-cell stage. The results show that there is a 2-f...

Legionella causes severe pneumonia in humans. The pathogen produces an array of effectors, which interfere with host cell functions. Among them are the glucosyltransferases Lgt1, Lgt2 and Lgt3 from L. pneumophila. Lgt1 and Lgt2 are produced predominately in the post-exponential phase of bacterial growth, while synthesis of Lgt3 is induced mainly in the lag-phase before intracellular replication...

N-linked protein glycosylation follows a conserved pathway in eukaryotic cells. The assembly of the lipid-linked core oligosaccharide Glc3Man9GlcNAc2, the substrate for the oligosaccharyltransferase (OST), is catalyzed by different glycosyltransferases located at the membrane of the endoplasmic reticulum (ER). The substrate specificity of the different glycosyltransferase guarantees the ordered...

Fringe proteins are beta1,3-N-acetylglucosaminyltransferases that modulate signaling through Notch receptors by modifying O-linked fucose on epidermal growth factor domains. Fringe is highly conserved, and comparison among 18 different Fringe proteins from 11 different species identifies a core set of 84 amino acids that are identical among all Fringes. Fringe is only distantly related to other...

AIMS Soils are rich, diversified environments where β-glucosidases abound because of their importance in organic matter degradation. The aim of this work was to discover new β-glucosidases by constructing two metagenomic DNA libraries from soil samples collected in winter and spring from a field of winter wheat. METHODS AND RESULTS Both libraries were screened on esculin-supplemented medium s...