Background Sperm preparation before assisted reproductive techniques is very important for obtaining successful fertilization and pregnancy. During ICSI technique, sperms are selected based on viability and morphology. But this selection did not guarantee integrity of sperm DNA. Therefore, novel sperm selection procedures are introduced for selection of sperm with low DNA damage. One of these p...

Gatica et al. (2013) report the development of a new device, the sperm selection assay (SSA), for enriching the content of high-quality, functionally competent cells in a human sperm sample. The device is technically simple, but exploits a sophisticated characteristic of many motile cells and organisms—their ability to direct their movement in response to the concentration gradient of a chemica...

BACKGROUND Density gradient is the preferred technique for sperm processing for ART. However, no study has examined sperm quality using different processing media simultaneously and under identical conditions. Therefore, we evaluated semen quality following sperm preparation by three commonly used commercially available density gradient media in a well-designed controlled trial. METHODS We ob...

AIM To 1) compare post-wash and post-thaw parameters of sperm processed with PureSperm density gradient technique and swim-up method; and 2) test the efficacy of two commonly available density gradient media PureSperm and ISolate. METHODS This prospective study used semen specimens from 22 patients. Specimens from nine patients were processed by both PureSperm density gradient and swim-up met...

Transient Receptor Potential Vanilloid (TRPV) 4 is one of the temperature-sensitive ion channels involved in temperature receptors, and it is known to be activated from 35 to 40ºC. Here we analyzed sperm motility function of Trpv4 knockout (KO) mouse in temperature-gradient conditions to elucidate the thermotaxis of mouse sperm and the involvement of TRPV4 in thermotaxis. The sperm were introdu...

OBJECTIVE:We evaluated the effectiveness of spermwashing using the discontinuous two-layer density gradient centrifugation method to remove antisperm antibodies attached to the sperm surface. METHOD: We prospectively enrolled sixty-six men with unexplained infertility who were seeking evaluation. Each patient delivered one semen specimen for the study. We determined antisperm antibody levels us...

conclusions albumin gradient method is the enrichment of y-bearing chromosome sperms. separation of spermatozoa by albumin gradient method, introduced by ericsson, is a suitable method for sex selection of male offspring. this technique is safe, because it neither requires toxic materials during sperm separation, nor involves embryo manipulation. objectives the study aimed to assess the separat...

although the mechanisms under which sex ratio is controlled in nature remain unknown, researchers have attempted to treat sperm in an effort to predetermine the sex of the offspring. the methods of sperm separation that have been repeatedly reported to isolate fractions rich in x- or y-chromosome bearing sperm are serum albumin gradient swim-up and flow cytometry. however, the ability of the se...

The ends of eukaryotic chromosomes contain specialized chromatin structures called telomeres, the length of which plays a key role in early human embryonic development. Although the effect of sperm preparation techniques on major sperm characteristics, such as concentration, motility and morphology have been previously documented, the possible status of telomere length and its relation with spe...

Chemotaxis refers to a process whereby cells move up or down a chemical gradient. Sperm chemotaxis is known to be a strategy exploited by marine invertebrates such as sea urchins to reach eggs efficiently in moving water. Less is understood about how or whether chemotaxis is used by mammalian sperm to reach eggs, where fertilization takes place within the confinement of a reproductive tract. In...