INTRODUCTION Anemia affects around 38.2% and 22% of pregnant women at a global and national level respectively. In developing countries, women start pregnancy with already depleted body stores of iron and other vitamins with significant variation of anemia within and between regions. OBJECTIVE To identify the determinants of anemia among pregnant mothers attending antenatal care in Dessie tow...

Inflammatory mediators produced during inflammatory response play an important role on pathological development of several chronic diseases. Although several dietary plants exhibited anti-inflammatory property, their impacts as a whole food has been rarely reported. The aim of the present study is to assess anti-inflammatory activity of an ethanol extract from a whole food namely “ready to eat ...

Environmental particles enter the chicken via several routes. Entry via the respiratory and cloacal routes likely activates immune responses. We studied the localization of simultaneous intratracheally and cloacally applied beads of 2 sizes in the chicken body in time, and when possible, semiquantified the amount of beads. Ten broiler hens, 3.5 wk of age, received 1.25 × 10(9) 1.0-µm beads and ...

The enhanced green fluorescent protein (EGFP) pEGFP-N1-P53 eukaryotic expression vector, which contains the human tumor suppressor p53, was constructed and transfected into chicken fibroblast cells and stage-X blastoderm to analyze the transfection efficiency. The complementary DNA of the human p53 gene was cloned by reverse transcription-polymerase chain reaction from human peripheral blood an...

Newcastle disease virus (NDV) is an important avian pathogen. We previously reported that NDV triggers autophagy in U251 glioma cells, resulting in enhanced virus replication. In this study, we investigated whether NDV triggers autophagy in chicken cells and tissues to enhance virus replication. We demonstrated that NDV infection induced steady-state autophagy in chicken-derived DF-1 cells and ...

The propagation of infectious laryngotracheitis virus (ILTV) has been described using primary cell cultures derived from chicken embryo liver and kidney or embryonated eggs, but these cultures use Specific Pathogen Free (SPF) eggs that are time and cost expensive. Since cell line cultures are easier to maintain in laboratory conditions, the growth of ILTV was evaluated in five different cell cu...

What many people do not realize is that the drug tamoxifen and the second generation. . I do not see any reason green tea would interact with Herceptin in an . Oct 25, 2011. Back in the 1970s, tamoxifen was designated as the first drug to. Green tea and melatonin are among the best-studied agents in this regard. To our knowledge, this study is the first to show the interaction of green tea with...

A method was developed to determine the survival of Campylobacter jejuni at specific sites on chicken skin, and this method was used to observe the survival of C. jejuni at various locations on the skin during storage. This method uses confocal scanning laser microscopy to visualize C. jejuni transformed with P(c)gfp plasmid (GFP-Campylobacter) and stained with 5-cyano-2,3-ditolyl tetrazolium c...

BACKGROUND RNA polymerase III (pol III) type 3 promoters such as U6 or 7SK are commonly used to express short-hairpin RNA (shRNA) effectors for RNA interference (RNAi). To extend the use of RNAi for studies of development using the chicken as a model system, we have developed a system for expressing shRNAs using the chicken 7SK (ch7SK) promoter. RESULTS We identified and characterised the ch7...

Preservation of ram semen has an impact on the exposure artificial conditions to spermatozoa which was followed by overproduction reactive oxygen species (ROS) and resulted in functional damages spermatozoa. This study aimed determine effect combination chicken, quail, duck egg yolks with green tea extract (GTE) quality Sapudi at 5°C storage. Ejaculates more than 70% motility were divided into ...