Photoactivatable fluorescent proteins are capable of dramatic changes in fluorescent properties in response to specific light irradiation. For example, they can be converted from cyan to green, or from green to red, or from nonfluorescent to a brightly fluorescent state. Several types of such proteins were developed recently, and some of them are already becoming popular tools to study protein ...

Indelible marking of pink bollworm (Pectinophora gossypiella) would assist in implementation of sterile insect technique-based pest control of this insect. Additionally, genetic manipulation through DNA-mediated genetic transformation also requires a reliable genetic marker. To this end, we injected DNA plasmids encoding the enhanced green fluorescent protein gene into pink bollworm pre-blastod...

Green fluorescent protein was purified from sonicated recombinant Escherichia coli and its mutant obtained after exposure to UV light. The latter overexpresses green fluorescent protein. The two-step procedure consisted of a two-phase aqueous extraction with PEG/salt and precipitation of the proteins from PEG phase by free Zn2+. The recoveries of green fluorescent protein were 73 and 83% in the...

Knowing that adenoviral vectors could initiate innate immunity, the ability of E1-deleted recombinant adenovirus (Ad-E1Δ) in induction of B7.1 and IL-2 molecules was studied. Methods: The expression of green fluorescent protein in C1498 cells following transfection of these cells with adenovirus green fluorescent protein vector confirmed the ability of adenovirus vectors in infecting the cells ...

In recent years diverse photolabeling techniques using green fluorescent protein (GFP)-like proteins have been reported1–7, including photoactivatable PA-GFP1, photoactivatable protein Kaede2, the DsRed ‘greening’ technique3 and kindling fluorescent proteins6,7. So far, only PA-GFP, which is monomeric and gives 100-fold fluorescence contrast, could be applied for protein tracking. Here we descr...