نتایج جستجو برای: griess assay
تعداد نتایج: 222159 فیلتر نتایج به سال:
BACKGROUND Performance characteristics of novel rapid drug susceptibility tests (DST) for Mycobacterium tuberculosis may change when moving from research to implementation in actual public health practice. We describe the performance characteristics of a direct, rapid DST when implemented in Lima, Peru. METHODS A district laboratory validated conventional proportions and nitrate reductase met...
The number of Alzheimer’s disease (AD) affected patients is increasing without any effective cure and the etiology remains to be understood. Inflammations, oxidative stress, Aβ, tauopathy are associated factors AD. Sodium butyrate (NaB) an HDAC inhibitor profoundly found neuroprotective. We have investigated neuroprotective effects NaB in SH-SY5Y cells stimulated with TNF-α/Aβ LPS-induced BV-2 ...
Over the past decade, cold atmospheric plasma (CAP) has undergone extensive research as a promising therapeutic approach in oncology, with different treatment methods and exposure configurations being investigated resulting various biological effects, most of them after long or durations. This study aimed to evaluate potential custom-made CAP generation source produce plasma-activated medium (P...
The aim of this study was to determine whether proteasome inhibitor MG132 treatment has any effect on endothelial progenitor cells (EPCs). Total mononuclear cells (MNCs) were isolated from peripheral blood by Ficoll density gradient centrifugation. EPCs were identified as adherent cells double positive for DiLDL-up-take and lectin binding by direct fluorescent demonstrated under a laser scannin...
Although ZnSe/ZnS quantum dots (QDs) have emerged as apparently less hazardous substitute to cadmium-based QDs, their toxicity has not been fully understood. Huge levels of ROS production and associated difficulties comprise the underlying reason for nanomaterial in cells. This will cause both immunotoxicity genotoxicity. In current work, Zinc Selenium/Zinc Sulphide (ZnSe/ZnS) QDs was synthesiz...
Oxidation of nitric oxide in aqueous solution to nitrite but not nitrate: comparison with enzymatically formed nitric oxide from L-arginine. A spectrophotometric assay for nitrate using NADPH oxidation by Aspergillus nitrate reductase. Measurement of nitrite and nitrate in biological fluids by gas chromatography mass spectrometry and by the Griess assay: problems with the Griess assay—solutions...
Purpose: To evaluate the anti-neuroinflammatory effects of Houttuynia cordata extract (H. cordata) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells, and its anti-oxidant properties. Methods: Anti-oxidant properties were evaluated by 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. Cell viability was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5diphenyl-tetrazolium b...
PURPOSE To investigate the effect of advanced glycation end products (AGE) on oxidative stress and cellular senescence in cultured human trabecular meshwork cells (HTMC). METHODS Primarily cultured HTMC were exposed to 0, 10, 50, 100, 200 µg/mL of glycated bovine serum albumin (G-BSA) for 5 days. Also co-exposed were L-arginine, sepiapterin, and antioxidant N-acetylcysteine (NAC). Cellular su...
Purpose: To investigate the effect of total glucosides of Paeonia lactiflora (TGPL) on nitric oxide (NO) production and its potential mechanism in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Methods: RAW264.7 cells were treated with 10 300 μg/ml TGPL and 1 μg/ml LPS. Cell survival was determined by MTT assay. NO level was determined by Griess reaction assay. Inducible NO synt...
If a vertex operator algebra V = ⊕n=0Vn satisfies dimV0 = 1, V1 = 0, then V2 has a commutative (nonassociative) algebra structure called Griess algebra. One of the typical examples of commutative (nonassociative) algebras is a Jordan algebra. For example, the set Symd(C) of symmetric matrices of degree d becomes a Jordan algebra. On the other hand, in the theory of vertex operator algebras, cen...
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