The use of a novobiocin-containing medium provided little benefit over observable quantitative growth on blood agar in detecting Staphylococcus saprophyticus in urine cultures.

This review article deals with a new element 'nipponium' reported by Masataka Ogawa in 1908, and with its scientific and science historical background. Ogawa positioned nipponium between molybdenum and ruthenium in the periodic table. From a modern chemical viewpoint, however, nipponium is ascribable to the element with Z=75, namely rhenium, which was unknown in 1908. The reasons for this corre...

The development and evaluation of a new selective medium (colistin-oxolinic acid-blood agar) for streptococci is described. Streptococci of medical and veterinary importance grew well on the medium. Gram-negative organisms, staphylococci. Bacillus spp., and coryneforms are all inhibited. It was concluded that the medium is valuable for the isolation of streptococci in pure culture from mixed fl...

A selective identification medium for Neisseria gonorrhoeae was devised by incorporating antibiotics and a mixture of sugars into a yeast extract agar. The results were compared with those using a standard combined selective/non-selective blood agar medium. Recovery rates of N gonorrhoeae from specimens from 208 patients cultured on both media were comparable, but the selective descriptive medi...

A previous paper (Eaton, 1934) described variants of the pneumococcus characterized by a tendency to undergo rapid spontaneous lysis under certain cultural conditions at 370C. These variants were produced by daughter-colony dissociation of smooth pneumococcus strains on blood agar at 250C. A further investigation of this method of dissociation revealed that a second and independent type of vari...

The carbohydrates of whole cells of group A, B, C, D, F, and G streptococci were analyzed with a highly sensitive gas chromatographic procedure. Characteristic chromatographic fingerprints were obtained for each group of streptococci grown in broth cultures or as single colonies on a blood agar plate. Rhamnose, glucose, and N-acetylglucosamine were major components of all the groups. Groups A, ...

No statistical differences were found in the recovery of group A streptococci from throat culture specimens after overnight incubation of blood agar plates in 5% CO2 compared with anaerobiosis. Anaerobic incubation required many more subcultures and resulted in considerably greater technical time and expense.

The isolation of beta haemolytic streptococci was compared using Columbia sheep blood agar with and without gentamicin in a final concentration of 5.5 micrograms per ml. The inclusion of gentamicin gave greatly improved results whenever the isolation of this organism in pure culture was complicated by overgrowth with Gram-negative bacilli or staphylococci.

Surgical site infections are one of the most important causes of healthcare associated infections (HCAI), accounting for 20% of all HCAIs. Surgical site infections affect 1% of joint replacement operations. This study was designed to assess whether theatre clothing is contaminated more inside or outside the theatre suite. Petri dishes filled with horse blood agar were pressed on theatre clothes...

Sabouraud dextrose agar with and without chloramphenicol (0.05 mg/mL) and cycloheximide (0.5 mg/mL) and incubated at 26C and 37C respectively. The cultures were examined twice weekly for a period of six weeks. The different types of colonies were subcultured on Sabouraud dextrose agar slants and pure cultures of the isolates were identified on the basis of microscopic morphology and cultural ch...