BACKGROUND Glucosamine (GlcN) used by patients with osteoarthritis was demonstrated to reduce pain, but the working mechanism is still not clear. Viscosupplementation with hyaluronic acid (HA) is also described to reduce pain in osteoarthritis. The synthesis of HA requires GlcN as one of its main building blocks. We therefore hypothesized that addition of GlcN might increase HA production by sy...

Hyaluronic acid drug conjugates can target anti-cancer drugs directly to tumor tissue for loco-regional treatment with enhanced bioavailability, local efficacy and reduced toxicity. In this study, the distribution and pharmacokinetics of hyaluronic acid carrier and a conjugated cisplatin anti-cancer drug were tracked by lanthanum (III) [La(III)] affinity tagging of the nanocarrier. The strong b...

bovine oocytes matured in culture were denuded from cumulus cells with hyaluronidase and by repeat pipetting trough a fine pasture pipete and were inseminated with frozen-thawed spermatozoa in a protein-free bo medium containing 5m m-caffeine, l0 mg heparin/ml and with or without polyvinyl pyrrolidone (pvp) and different concentrations of hyaluronic acid. when oocytes were inseminated in the ab...

PURPOSE This study was performed to separately assess the aqueous flow applied with hyaluronic acid, and the behaviour of hyaluronic acid itself on the ocular surface. METHODS Two different fluorescent dyes, fluorescein sodium dissolved in 0.1% hyaluronic acid (HA) solution and 0.1% fluorescein conjugated with hyaluronic acid (F-HA) dissolved in saline, were used. A volume of 20 mul of tested...

The 1C6 monoclonal antibody to the hyaluronic acid-binding region weakly stained a 65-kD component in immunoblots of the chondroitin sulfate proteoglycans of brain, and the 8A4 monoclonal antibody, which recognizes two epitopes in the polypeptide portion of link protein, produced strong staining of a 45-kD component present in the brain proteoglycans. These antibodies were utilized to examine t...

The effects of hyaluronate on the morphology, motile and social behaviour of embryonic chick heart fibroblasts were examined in the presence or absence of hyaluronate binding proteins (HABP). HABP were inserted onto fibroblasts after treating cells with 0.2 M-urea. Such treatment increased the binding of [125I]HABP to cell monolayers: at saturation, binding was increased threefold compared to u...

The protein-keratan sulfate core of bovine nasal cartilage proteoglycan was purified by affinity chromatography on a column of immobilized hyaluronic acid. The hyaluronic acid was immobilized by reaction with a hydrazido-alkyl derivative of Sepharose in the presence of borohydride. Proteoglycan was digested with chondroitinase ABC and the entire mixture was passed over a column of the Sepharose...

The purposes of this study are to characterize the binding of hyaluronic acid (HA) to mouse T lymphoma cells, to measure changes in intracellular Ca2+ after HA binding, to elucidate the interaction between the HA receptor, GP85(CD44), and ankyrin in the membrane skeleton, and finally to correlate these events with HA receptor patching/capping and cell adhesion to HA. First, we established an in...

Hyaluronic acid (HA or hyaluronan) is a natural, high molecular weight, linear polysaccharide composed of alternating N-acetyl glucosamine and glucuronic acid disaccharide units. HA is ubiquitous in human and animal tissues. It is found in the vitreous body, in the extracellular matrix of soft connective tissues, and in the synovial fluids. By supplementing the body’s level of glycosaminoglycan...

In the present study, we propose Hyaluronic acid - 2-Deoxy-D-Glucose (HA-2DG) conjugate as a novel drug for treatment of COVID-19. silico molecular docking studies HA-2DG and (2DG) against four different SARS-CoV-2 viral protein (Mpro, RdRp, PLpro S protein) revealed that showed better binding affinity (-6.2, -7.2, -7.0 6.4 Kcal/mol) with all screened SASR-CoV-2 targets than antimetabolite 2DG ...