Single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods were compared for the detection of Myxobolus cerebralis. Parallel tests were conducted on a total of 1743 free-ranging and 400 hatchery-reared salmonids. Concurrent results were found in 84.6% of the free-ranging fish samples, and 83.5% of the hatchery samples. PCR identified M. cerebralis more frequently than ...

For over a century, harvests of eastern oysters (Crassostrea virginica) have been declining in the northeast. This trend has been particularly acute in the mid-Atlantic region where two diseases, MSX and Dermo, have caused catastrophic mortalities. With the reduction in natural populations, growers have increasingly turned to the use of hatchery technology for annual supplies of seed oysters. T...

Early-rearing salmonids in Ontario Ministry of Natural Resources (OMNR) fish hatcheries have been consistently affected by bacterial gill disease (BGD) (causative agent: Flavobacterium branchiophilum) for many years. Separate retrospective epidemiological investigations of BGD treatments at two OMNR fish hatcheries (Hatcheries A and B) for the 1999 production year were conducted using on-site h...

We examined the patterns of habitat-specific mortality for newly settled red drum (Sciaenops ocellatus) using an experimental mesocosm approach. Experiments were designed to analyze prey vulnerability and fish rearing-type (wild-caught or hatchery-reared) in estuarine habitats of varying structural complexity including marsh (Spartina alterniflora Loisel), oyster reef (Crassostrea virginica Gme...

Starry flounder (Platichthys stellatus) is an important sport and food fish found around the margins of the North Pacific. Aquaculture production of this species in Korea has increased because of its commercial value. Microsatellite DNA markers are a useful DNA-based tool for monitoring the genetic variation of starry flounder populations. In this study, 12 polymorphic microsatellite DNA marker...

A 10 week feeding trial was carried out to evaluate the replacement value of hatchery wastes for fish meal in the diet of laying Japanese quail (Coturnix coturnix japonica). 108 female Japanese quails (36 per treatment) were randomly allocated into 3 dietary treatments A, B and C which are fish meal (FM), whole hatchery waste meal (WHWM) and shelless hatchery waste meal (SHWM) based diets respe...

While two independent Chinook populations have been identified within WRIA 8 by the PSTRT, Marshall (2000) indicated that there might be enough difference between the North Lake Washington Chinook (Bear/Cottage Lake creeks) and fish returning to Issaquah Creek to consider them separate from one another. WRIA 8 would then contain three distinct Chinook populations (Cedar River, Bear/Cottage Lake...