purpose: in order to express human granulocyte-macrophage colony stimulating factor (hgm-csf) under heat shock. materials and methods: two expression plasmids were constructed based on pbc(sk) plasmid. the expression cassettes in the two plasmids are equipped with a 75 base pair fragment, derived from the pl promoter of the bacteriophage lambda (λ). the plasmids also contain a temperature muta...

In this issue of Molecular Cell, Di Giammartino et al. (2012) identify a new function for PARP1 in the post-transcriptional regulation of mRNAs via ADP-ribosylation of poly(A) polymerase, a mRNA 3′ processing enzyme. Poly(ADP-ribose) polymerase 1 (PARP1, also known as ARTD1) is an abundant nuclear protein that covalently modifies target proteins with poly(ADP-ribose) (PAR) using NAD+ as a subst...

background: the homologous recombination (hr) is one of the conventional cloning methods for the production of recombinant dna. it is a quick method for in vivo dna cloning without using the high cost restriction enzymes. a few modifications in the cloning procedure can increase the efficiency of this method. m aterials and methods: in this study, effect of heating on the rate of the igg1 heavy...

to design the equipment and facilities for preservation, storage and processing of pomegranate, it is necessary to know the specific heat and thermal conductivity of the fruit components. the specific heat of exocarp, mesocarp and of seeds of pomegranate were assessed at three moisture content levels (30, 50 and 76% for seed and mesocarp; 15, 35 and 52% for exocarp) and at four temperature leve...

The effective parameters that influence in situ cast ferroTic composites were investigated. Centrifugal casting of specimens was carried out using ceramic & metallic molds. OM, SEM and XRD techniques were used to examine the existence of flows in the specimens. Results show that the control of chemical composition, processing, cooling rate and heat treatment has &#10a promising effect on the qu...

Laser materials processing with ultra-short pulses allows very precise and high quality results with a minimum extent of the thermally affected zone. However, with increasing average laser power and repetition rates the so-called heat accumulation effect becomes a considerable issue. The following discussion presents a comprehensive analytical treatment of multi-pulse processing and reveals the...

The quantity of ionizing radiations needed to sterilize canned foods generally impairs both their nutritive qualities and palatability (Hannan, 1955; Schultz et al., 1956). However, thermal processes also lower these qualities in canned foods (Stumbo, 1949). In both instances, the extent of damage increases with the severity of the treatments. Consequently, the heat processing schedules that ar...

HARRIS, R. J. C. 1954 Biological applications of freezing and drying. Academic Press, Inc., New York, New York. HECKLY, R. J. 1955 Rapid and precise measurement of moisture in biological materials. Science, 122, 760-761. MERYMAN, H. T. 1956 Mechanics of freezing in living tissues. Science, 124, 515-521. MILES, A. A., MISRA, S. S., AND IRWIN, J. 0. 1938 The estimation of bactericidal power of th...

The effective parameters that influence in situ cast ferroTic composites were investigated. Centrifugal casting of specimens was carried out using ceramic & metallic molds. OM, SEM and XRD techniques were used to examine the existence of flows in the specimens. Results show that the control of chemical composition, processing, cooling rate and heat treatment has a promising effect on the qual...