Hybrid genes containing the 5' region of Drosophila heat shock protein (hsp) genes, ligated to the herpesvirus thymidine kinase (tk) gene, were transfected into Drosophila line S3 cells. Constructions containing sequences upstream from hsp70, or from any of the small hsp genes, showed heat-inducible tk expression. Ecdysterone-inducible tk expression was seen only in transfections with small hsp...

Under conditions of heat shock at 42 degrees C, mRNAs of HSP (heat shock protein) genes are exported out of the nucleus, whereas bulk poly(A)(+) (polyadenylated) mRNA shows a nuclear accumulation in Saccharomyces cerevisiae. Such a selective mRNA export seems an efficacious strategy of yeast cells to adapt rapidly to stress. Although ethanol stress (10%, v/v) as well as heat shock blocks the ex...

Exposure of NIH3T3 cells to elevated temperatures induces the phosphorylation and activation of mitogen-activated protein (MAP) kinases [or extracellular signal-regulated kinases (ERKs)]. To investigate the significance of MAP kinase activation by heat shock, we examined the effect of inhibiting the activity of MAP kinase on heat shock protein 70 (hsp 70) expression. Overexpression of a dominan...

Heat shock protein/Thermotolerance/Cycloheximide The relation of heat shock protein (HSP) synthesis and development of thermotolerance was compared in Chinese hamster V79 cells of low, and mouse L cells of high, thermosensitivities. Thermotolerance was induced by step-up heating (42•Ž, 2 hr and 44•Ž). Cycloheximide did not inhibit the development of ther motolerance in V79 cells. In contrast, n...

BACKGROUND The heat shock response (HSR) is an ancient and highly conserved program of stress-induced gene expression, aimed at reestablishing protein homeostasis to preserve cellular fitness. Cells that fail to activate or maintain this protective response are hypersensitive to proteotoxic stress. The HSR is mediated by the heat shock transcription factor 1 (HSF1), which binds to conserved hea...

Besides heme deficiency, protein synthesis in rabbit reticulocyte lysates becomes inhibited upon exposure to a variety of agents that mimic conditions which induce the heat shock response in cells. This inhibition has been demonstrated to be due primarily to the activation of the heme-regulated eIF-2 alpha kinase (HRI) which causes an arrest in the initiation of translation. In this report, the...

introduction: the aim of this study was to investigate the effect of the hsp-70 (heat shock protein-70) induction in the lysate of heat shocked tumor cells on splenocyte proliferation, nitric oxide production by peritoneal macrophages, and fibrosarcoma tumor size reduction in balb/c mice. material and methods: wehi 164 cells (mouse fibrosarcoma cell line) were injected subcutaneously into the r...

Synthesis of mRNA in trypanosomes involves an apparent trans-splicing reaction whereby a common 39-nucleotide mini-exon sequence is joined to the protein-coding exon of a mRNA precursor. We have previously shown (Muhich, M. L., and Boothroyd, J. C. (1988) Mol. Cell. Biol. 8, 3837-3846) that the trans-splicing pathway of Trypanosoma brucei is sensitive to disruption by severe heat shock. Here we...

By using a map of the unique region of DNA encoding the fur small heat-shock proteins of Drosophila melanogaster (hsp 22, hsp 23, hsp 26, and hsp 28), and a simple mapping technique, the positions of the DNase I hypersensitive sites of chromatin in the vicinity of these genes have now been determined. The major chromatin-specific sites occur at the 5' ends of each of the four heat-shock protein...

Molecular chaperones are required to maintain the proteome in a folded and functional state. When challenges to intracellular folding occur, the heat shock response is triggered, leading to increased synthesis of a class of inducible chaperones known as heat shock proteins (HSP). Although HSP synthesis is known to undergo a general decline in most cells with aging, the extent of this process va...