1. By using a monolayer of tissue culture cells, JTC-3, JTC-3’, JTC-3” and HeLa cells, the assays of neotetrazolium (NT) reduction at the cell level have been conducted on the cells without detaching the cells from the culture glass vessel wall. 2. It has been found that the activity of endogenous NT reductase is extremely high in the living tissue culture cells. The endogenous NT reductase act...

BACKGROUND The aim of this study was to compare invasive and non-invasive strains of Shigella flexneri isolated from Tehran by a 120 kDa protein band by SDS-PAGE, electron microscopy of cell culture and Congo red dye methods. METHODS S. flexneri strains were isolated by standard bacterial methods from fecal specimens of children attending to the 3 children's hospitals. Phenotype analysis for ...

Puck and his colleagues (1956, 1957) classified tissue-cultured cells into 2 types, epithelioid (Ep) and fibroblast-like (Fb) cells, and reported that their morphological features were constant through many passages so that they could be supposed to be under genetic control. The HeLa strain derived from a human cervical carcinoma (Gey et al., 1952) is a typical cell line belonging to Ep-type of...

Radioprotector/Cysteamine /Mitosis/HeLa Mitotic cells disappeared nearly completely within 60 min after the addition of 30 mM cysteamine in HeLa S-3 cell culture, while the control culture contained mitotic cells with the frequency of 3.4%. Inhibition of mitosis became gradually irreversible when the treatment was extended over 60 min, but cytolysis occurred much more slowly. Ethanolamine exhib...

Infectious tissue culture fluids of the majority of serotypes of adenovirus at low dilutions detach HeLa or KB cells from glass surfaces within a few hours after inoculation. A reproducible method for testing cell detachment was devised. The factor present in infectious tissue culture fluids and responsible for cell detachment is trypsin-sensitive and non-dialyzable; it is smaller and more resi...

BACKGROUND This study was performed to induce conversion of RH strain tachyzoites of Toxoplasma gondii to bradyzoites by pH changing of the culture medium. METHODS HeLa cell monolayers were infected at a 1:1 tachyzoite to cell ratio. Four hours after infection, the culture medium was removed and replaced with culture medium and 5% FCS, adjusted to pH 8 with NaOH. The culture was maintained at...

background: our previous studies revealed an inhibitory effect of icd-85 (venom derived peptides) on mda-mb231 and hl-60 cell lines, through induction of apoptosis. the purpose of this study was to investigate apoptosis-induced mechanism on hela and mrc-5 cells by icd-85 through activation of caspase-8. methods: cell viability, cytosolic enzyme lactate dehydrogenase (ldh) and cell morphology we...

0022-0728/$ see front matter 2009 Elsevier B.V. A doi:10.1016/j.jelechem.2009.01.002 * Corresponding author. Tel.: +1 512 471 3761; fax E-mail address: [email protected] (A.J. Bard) The viability and activity of HeLa cells were probed using scanning electrochemical microscopy (SECM). The feedback generated by HeLa cells during scanning depends on the electrochemical mediator. Living HeLa c...

celecoxib is a non-steroidal anti-inflammatory drug (nsaid) developed as a selective inhibitor of cyclooxygenase-2 (cox-2) for the treatment of rheumatoid arthritis disease. recently some other mechanisms have been identified for anti cancer activity of these agents including induction of apoptosis, inhibition of tumor vascularization, stimulation of antitumor immune responses and inhibition of...

Allium hirtifolim (Persian Shallot) belongs to Allium genus (Alliaceae family). We investigated the in vitro effects of chloroformic extract of A. hirtifolium and its Allicin on the proliferation of HeLa (cervical cancer), MCF7 (human, caucasion, breast, adenocarcinoma) and L929 (mouse, C3H/An, connective) cell lines. Our results showed that components of A. hirtifolium might inhibit proliferat...