Development of a stool antigen immunoassay to detect Helicobacter pylori infection requires monoclonal antibody against the specific antigen. Alkylhydroperoxide reductase (AhpC) of Helicobacter pylori has been described as a specific and unique enzyme for H. pylori and therefore, both H. pylori AhpC and Anti-AhpC could be useful in the development of serologic and stool antigen tests, for detec...

background: some antigens of h. pylori are excreted into the stool of infected people. these antigens can be used to detect the infection by immunoassays such as elisa. our aim was to identify these antigens by immunoblotting and affinity chromatography techniques.   methods: four different antigenic preparations, namely, whole cell sonicate (wcs), outer membrane proteins (omps), cytoplasmic an...

BACKGROUND The purpose of this study is to compare validity of 5 diagnostic tests of helicobacter pylori with each other: stool antigen test, urea breath test (UBT), rapid urease test (RUT), serology and histology. METHODS A total of 94 patients who had indication of endoscopy entered the study. All of the 5 tests were performed for each patient. When the results of at least 2 tests were posi...

background and aims: helicobacter pylori (h. pylori) is the microorganism that infects nearly half of word's population. there are several invasive and non-invasive methods for diagnosis of infection. the main objective of this study was to evaluate antigen of h. pylori in feces with h. pylori stool antigen (hpsa) test and comparison with rapid urease test (rut) in the patients suspected t...

BACKGROUND Hemodialysis is the most common method of renal replacement therapy for treatment of acute and chronic kidney failure. Helicobacter pylori (H. pylori) plays a major role in development of peptic ulcer, gastric neoplasms, and lymphoma as well as increased risk of cardiovascular disorders in hemodialysis patients. OBJECTIVES In this study the diagnostic values of noninvasive tests [i...

BACKGROUND/AIMS We aimed to determine the etiology of patients with duodenal and gastric ulcers. METHODS 140 patients diagnosed with peptic ulcer between April 2002-2009 were enrolled in this prospective study. Two biopsy specimens were collected from the antrum and corpus for histology and one for rapid urease testing, and stool samples were analyzed for Helicobacter pylori antigen. Serum ca...

Although several invasive and noninvasive tests have been developed for the diagnosis of Helicobacter pylori infection, all of the tests have their limitations. We conducted a study to investigate and compare the suitability of rapid urease test (RUT), serology, histopathology and stool antigen tests with polymerase chain reaction (PCR) for detection of H. pylori, and correlate the diagnostic m...

OBJECTIVE To compare the usefulness of 4 commonly used tests in the diagnosis of Helicobacter pylori H. pylori infection in Saudi patients with dyspepsia. METHODS Patients presenting with dyspepsia at the gastroenterology clinic of Aseer Central Hospital, Abha, Kingdom of Saudi Arabia between October 2005 to May 2006, who consented to participate in the study were enrolled. Patients who recei...

background : animal models have been proven useful in elucidating the details of interaction between pathogenic bacteria and their human hosts, in addition to assessing the efficacy of therapeutic compounds and vaccines. in this study, we investigate the colonization of helicobacter pylori ( h. pylori ) in experimentally-infected guinea pigs over a six-month period. materials and methods: a bac...

Helicobacter pylori gastritis infection rate increases with age. Higher rates have however been reported among young people in the developing countries of the world. The infection however has rarely been reported in infants, especially in Africa. This case series describes three cases of Helicobacter pylori gastritis infection as diagnosed in three infants. The goal is to raise the suspicion in...