Two phytohemagglutinins (strongly hemagglutinating Maockia amurensis hemagglutinin and strongly mitogenic Maockia amurensis hemagglutinin (MAH and MAM)), which have mitogenic activity against human peripheral lymphocytes, have been purified from the seeds of Maackia amurensis by affinity chromatography on porcine thyroglobulin glycopeptides-Sepharose columns followed by SESephadex chromatograph...

UNLABELLED Zoonotic avian influenza virus infections may lead to epidemics or pandemics. The 1918 pandemic influenza virus has an avian influenza virus-like genome, and its H1 hemagglutinin was identified as a key mammalian virulence factor. A chimeric 1918 virus expressing a contemporary avian H1 hemagglutinin, however, displayed murine pathogenicity indistinguishable from that of the 1918 vir...

UNLABELLED Influenza viruses expressing chimeric hemagglutinins (HAs) are important tools in the quest for a universal vaccine. Using cryo-electron tomography, we have determined the structures of a chimeric HA variant that comprises an H1 stalk and an H5 globular head domain (cH5/1 HA) in native and antibody-bound states. We show that cH5/1 HA is structurally different from native HA, displayi...

This study was trying to predict the mutations in H1 hemagglutinins of influenza A virus from North America including the predictions of mutation position, the predictions of would-bemutated amino acids and the predictions of time of occurrence of mutations. The results paved a possible way for accurate, precise and reliable prediction of mutation in proteins from influenza A virus.

Erythrocytes modified to different extents with periodate were used in hemagglutination assays to investigate the binding properties of antihemagglutinin monoclonal antibody-selected antigenic variants of X-31 influenza virus. The results allowed differentiation of groups of variants and are discussed in relation to the nature of the amino acid substitutions in the variant hemagglutinins and th...

The standardization and quantitative evaluation of an assay for myxoviruses, based on the enumeration of individual infected clone 1-5C-4 cells manifesting hemadsorption within 24 h of infection, are described. Hemadsorption was detectable earlier than immunofluorescence in infected cells or hemagglutinins in culture medium. The relationship between virus concentration and cells exhibiting hema...

b a ckground: the role of lectins is important in interaction between pathogens and mosquito vectors. this study was performed to identify agglutinin activities of protein molecules on the midgut of culex pipiens . m e t hods: c u lex pipiens was reared in insectray condition and the midguts of males and females (blood fed and un- fed) were dissected separately in tris-hcl buffer. the extracts ...