We have developed a Quantitative Light Absorption Analysis (QLAA) method to rapidly estimate human lymphocyte concentrations isolated from small volumes of whole blood. Measurements of the light absorption analysis were calibrated for lymphocyte concentration levels using a hemocytometer. To validate the QLAA system, blood samples were collected from 17 healthy donors and lymphocyte absorption ...

on quantitation by use of a hemacytometer. Depending upon the types of cells used and the experience of the workers in various laboratories, the dilutions of the packed volumes of cells may range from 1:100 to 1:600. After 3 to 5 days of incubation of the initial cell suspension, the usual subsequent steps are the removal and discarding of all unattached cells and replacement of the original me...

With nearly 5000 areas examined, the comparison of a Poisson distribution to the distribution of leucocytes in a hemocytometer chamber at both 1:100 and 1:25 dilutions indicated a poor fit (P= <0.0001), a non-random distribution. In a series of leucocyte determinations from a young, male university student comparing 28 counts from 18 mm (both chambers) with 28 from 9 mm, and 28 from 4 mm (the u...

OBJECTIVE To assess the accuracy of the Hemacytometer (Hausser Scientific, Horsham, PA), Makler (Sefi-Medical Instrument, Haifa, Israel), Cell-VU (Millennium Sciences Inc., New York, NY), and Micro-Cell chambers (Conception Technologies, San Diego, CA) counting chambers. DESIGN A solution containing a known concentration of latex beads was used as the standard to perform counts on the four di...

The original hemocytometer leukocyte adherence inhibi tion technique has been used to study immunological reactivity of patients with a variety of tumors. Cellular activity was closely correlated with exposure to tumor of the appropriate type. Specificity and sensitivity in terms of having few false positives and false negatives were of the order of 90% or greater, and repeated testing of the s...

the objective of the present study was to examine the effects of chilling period on semen quality of sannan and jamunapari. semen of goat breeds were collected with artificial vagina and chilled at 5-9˚c. volume, colour and ph were determined on fresh semen. after dilution, microscopic examination was performed for the progressive motility and sperm viability. diluted semen of both breed was co...

This Application Note describes an alternative process for bacterial cell counting using on-chip fl ow cytometry with the Agilent 2100 Bioanalyzer system. This procedure is described with three different kinds of prokaryotes: Escherichia coli (gram-negative, rod-shaped), Pseudomonas aeruginosa (gram-negative, rod-shaped), and Staphylococcus aureus (gram-positive, coccal). As a reference for bac...

Invertebrate organisms mostly have innate immune system. Hemocytes are the cells of immunity in invertebrates. Our study was designed to observe effect malathion insecticide on system, function and hemocytes Drosophila melanogaster. A hemocytometer used for total count which showed a reduced percentage live (from 91.63% 44.57%) due treatment. For differential count, hemolymph spread as smear cl...

Idiopathic thrombocytopenia purpura (ITP) is an autoimmune disorder characterized by isolated thrombocytopenia and mucocutaneous bleeding. The former has a primary role in the diagnosis and treatment of ITP. One of the leading causes of miscounted platelets in patients who suffer from ITP is giant platelets which may indicate factitiously a severe false thrombocytopenia. Here, a case of macroth...