First detected by Chang et al. (1994) in Kaposi sarcomas associated with the acquired immune deficiency syndrome (AIDS) (see IARC, 1996) by representational difference analysis, this virus was termed Kaposi-sarcoma-associated herpesvirus, KSHV. KSHV is also associated with primary effusion lymphoma and some cases of multicentric Castleman disease (see Section 2). In keeping with the systematic ...

Murine herpesvirus 68 (MHV-68) is a naturally occurring herpesvirus of small free-living rodents. In order to facilitate the molecular characterization of the virus genome, a library of cloned restriction fragments has been produced and restriction enzyme cleavage maps deduced for the enzymes BamHI, EcoRI and HindIII. The MHV-68 genome comprises a region of unique DNA of approximately 118 kbp w...

Adenovirus mutants carrying a defective E1A gene, such as dl312, are unable to express any of the early viral genes upon infection of HeLa cells. However, efficient expression of the other early adenovirus genes was obtained when dl312-infected HeLa cells were coinfected with pseudorabies virus, a herpesvirus. By employing a temperature-sensitive pseudorabies mutant (tsG1) it was demonstrated t...

Primate gamma-2 herpesviruses (rhadinoviruses) have so far been found in humans (Kaposi's sarcoma-associated herpesvirus [KSHV], also called human herpesvirus 8), macaques (Macaca spp.) (rhesus rhadinovirus [RRV] and retroperitoneal fibromatosis herpesvirus [RFHV]), squirrel monkeys (Saimiri sciureus) (herpesvirus saimiri), and spider monkeys (Ateles spp.) (herpesvirus ateles). Using serologica...

Four human herpesviruses were discovered in a eight-year period between 1986 and 1994. This exciting era of virus discovery was driven in part by the search for HIV and HIV-related diseases coupled with the development of new molecular tools such as PCR, automated Sanger sequencing, and subtractive hybridization. Three of these viruses, human herpesvirus 6A (HHV-6A) and human herpesvirus 6B (HH...

An electron microscopic study (EM) was performed on herpesvirus-infected cells in epidermal lesions of larvae of the Japanese flounder Paralichthys olivaceus to document the ultrastructure of the lesions. Epithelial cells were the primary cell type infected with the herpesvirus, and showed nuclei containing nucleocapsids, vesicles containing enveloped virions in the cytoplasm, and the release o...

conclusion there was no association between hhv-8 and mf or lpp in iranian population. this is in accordance with most of the reports from different countries. results the investigated samples consisted of 13 patient with mf, 10 patients with lpp, and 23 patients with chronic dermatitis; the diagnosis of these skin conditions were confirmed histopathologically. the rt-pcr could not detect hhv-8...

Identification of herpesvirus in biological material is usually carried out by real-time PCR. With the aim to classify the strain of virus identified, real-time PCR must be often supported by time-consuming capillary electrophoresis sequencing analysis. Here we provide a protocol for the rapid and reliable identification of 5 closely related herpesviruses by PyroMark Q24 sequencing system. Pyro...